{"created":"2023-05-15T16:41:33.987572+00:00","id":16232,"links":{},"metadata":{"_buckets":{"deposit":"ac17b363-ed1c-44c5-b377-2f706ee36bdf"},"_deposit":{"created_by":2,"id":"16232","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16232"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016232","sets":["10:11"]},"author_link":["61123","61124","61125","61122","61126","61121"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"479","bibliographicPageStart":"467","bibliographicVolumeNumber":"145","bibliographic_titles":[{"bibliographic_title":"Journal of biochemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"D-3-Hydroxybutyrate dehydrogenase (HBDH) from Pseudomonas fragi showed a strict stereospecificity to the d-enantiomer of 3-hydroxybutyrate (d-3-HB) as a substrate. The l-enantiomer acts as a competitive inhibitor, with a K(i) value comparable to the K(m) value for d-3-HB. We have determined the crystal structures of the ternary complex of HBDH-NAD(+)-l-3-HB and the binary complex of HBDH-NAD(+). The former structure showed a so-called closed-form conformation, which is considered an active form for catalysis, while the latter stayed mostly in a open-form conformation. The determined structures along with the site-directed mutagenesis confirmed the substrate recognition mechanism that we proposed previously. The hydrogen bonding interaction between Gln196, located in the moving helix, and the carboxyl group of the substrate/inhibitor is important for the stable ternary complex formation. Finally, the crystal structures of the Thr190 mutants, T190S and T190A, indicate that the Thr190 is a key residue for the open-closed conformational change. T190S retained 37% of the activity. In T190A, however, the activity decreased to 0.1% that of the wild-type enzyme. Fixing the position of the hydroxyl group of Thr190 to form hydrogen bonds to the pyrophosphate moiety and the carboxamide of NAD(+) seems to be a significant factor for the open-closed conformational change.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of biochemistry, 145(4), pp.467-479; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19122202","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvn186","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c) The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1756-2651","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakashima, Kanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Kiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakajima, Yoshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamazawa, Ryuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyakawa, Syunsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimoto, Tadashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JB145_467.pdf","filesize":[{"value":"899.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JB145_467.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16232/files/JB145_467.pdf"},"version_id":"652358e4-0332-4f33-9c39-158403c09d97"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"hydroxybutyrate dehydrogenase","subitem_subject_scheme":"Other"},{"subitem_subject":"short-chain dehydrogenase/reductase family","subitem_subject_scheme":"Other"},{"subitem_subject":"crystal structure","subitem_subject_scheme":"Other"},{"subitem_subject":"conformational change","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate recognition","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Closed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Closed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor."}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-06"},"publish_date":"2009-11-06","publish_status":"0","recid":"16232","relation_version_is_last":true,"title":["Closed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:52:53.105384+00:00"}