@article{oai:nagasaki-u.repo.nii.ac.jp:00016237, author = {Lee, Jeong-Hoon and Kodama, Keita and Kume, Gen and Oyama, Masaaki and Katayama, Satoshi and Takao, Yuji and Horiguchi, Toshihiro}, issue = {2}, journal = {Fisheries Science}, month = {Feb}, note = {To find an appropriate method for age determination in the marbled sole Pseudopleuronectes yokohamae in Tokyo Bay, Japan, sagittal otoliths of 1,343 individuals were observed by surface-reading and cross-section methods and the results were compared. Opaque zones occurred once a year and were regarded as annuli in both methods. The surface-reading method sometimes provided a lower count of the number of annuli than the cross-section method, and the frequency of this discrepancy was highest in older fish (males above 5 years, females above 4 years). The oldest female fish was estimated to be age 10 years by the cross-section method but 8 years by the surface-reading method. The cross-section method could provide a more accurate estimate of age and is therefore likely to be indispensable to estimations of longevity. In contrast, the surface-reading method is superior in terms of cost and time efficiency but is likely to underestimate the ages of older fish. However, growth equations based on age estimated by the surface-reading method were sufficiently accurate if males ?5 years and females ?4 years were combined as specific, single age groups of 5+ and 4+, respectively., Fisheries Science, 75(2), pp.379-385; 2009}, pages = {379--385}, title = {Comparison between surface-reading and cross-section methods using sagittal otolith for age determination of the marbled sole Pseudopleuronectes yokohamae}, volume = {75}, year = {2009} }