@article{oai:nagasaki-u.repo.nii.ac.jp:00016271,
 author = {Rahman, Laiq ur and Kouno, Hitomi and Hashiguchi, Yuya and Yamamoto, Hirobumi and Narbad, Arjan and Parr, Adrian and Walton, Nicholas and Ikenaga, Toshihiko and Kitamura, Yoshie},
 issue = {20},
 journal = {Bioresource technology},
 month = {Oct},
 note = {As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14% on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones., Bioresource technology, 100(20), pp.4836-4842; 2009},
 pages = {4836--4842},
 title = {HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls.},
 volume = {100},
 year = {2009}
}