{"created":"2023-05-15T16:41:35.650095+00:00","id":16271,"links":{},"metadata":{"_buckets":{"deposit":"01b2295b-9f95-461e-94ae-8058295a8043"},"_deposit":{"created_by":2,"id":"16271","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16271"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016271","sets":["52:56"]},"author_link":["61308","61306","61302","61300","61305","61303","61307","61301","61304"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"20","bibliographicPageEnd":"4842","bibliographicPageStart":"4836","bibliographicVolumeNumber":"100","bibliographic_titles":[{"bibliographic_title":"Bioresource technology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14% on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Bioresource technology, 100(20), pp.4836-4842; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier BV"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19457657","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.biortech.2009.04.035","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2009 Elsevier Ltd All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11522750","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09608524","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1873-2976","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Rahman, Laiq ur"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kouno, Hitomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashiguchi, Yuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Hirobumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narbad, Arjan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Parr, Adrian"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Walton, Nicholas"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikenaga, Toshihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitamura, Yoshie"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"BT100_4836.pdf","filesize":[{"value":"750.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BT100_4836.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16271/files/BT100_4836.pdf"},"version_id":"bb0081ce-3927-47ed-bafc-3f175e0d5c34"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Beta vulgaris","subitem_subject_scheme":"Other"},{"subitem_subject":"Metabolic engineering","subitem_subject_scheme":"Other"},{"subitem_subject":"p-Hydroxycinnamoyl-CoA hydratase/lyase","subitem_subject_scheme":"Other"},{"subitem_subject":"p-Hydroxybenzoic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell wall","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls."}]},"item_type_id":"2","owner":"2","path":["56"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-09"},"publish_date":"2009-09-09","publish_status":"0","recid":"16271","relation_version_is_last":true,"title":["HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:51:11.414290+00:00"}