{"created":"2023-05-15T16:41:36.801747+00:00","id":16298,"links":{},"metadata":{"_buckets":{"deposit":"fb4e1ba8-0363-4601-aa7b-51077ec729ed"},"_deposit":{"created_by":2,"id":"16298","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16298"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016298","sets":["27:28"]},"author_link":["61477","61488","61480","61484","61478","61481","61487","61483","61479","61486","61485","61482"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-09-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"38","bibliographicPageEnd":"34966","bibliographicPageStart":"34959","bibliographicVolumeNumber":"277","bibliographic_titles":[{"bibliographic_title":"The Journal of biological chemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"At the primary structure level, the 90-kDa heat shock protein (HSP90) is composed of three regions: the N-terminal (Met(1)-Arg(400)), middle (Glu(401)-Lys(615)), and C-terminal (Asp(621)-Asp(732)) regions. In the present study, we investigated potential subregion structures of these three regions and their roles. Limited proteolysis revealed that the N-terminal region could be split into two fragments carrying residues Met(1) to Lys(281) (or Lys(283)) and Glu(282) (or Tyr(284)) to Arg(400). The former is known to carry the ATP-binding domain. The fragments carrying the N-terminal two-thirds (Glu(401)-Lys(546)) and C-terminal one-third of the middle region were sufficient for the interactions with the N- and C-terminal regions, respectively. Yeast HSC82 that carried point mutations in the middle region causing deficient binding to the N-terminal region could not support the growth of HSP82-depleted cells at an elevated temperature. Taken together, our data show that the N-terminal and middle regions of the HSP90 family protein are structurally divided into two respective subregions. Moreover, the interaction between the N-terminal and middle regions is essential for the in vivo function of HSP90 in yeast.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Biological Chemistry, Vol. 277, Issue 38, 34959-34966, September 20, 2002","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"12121981","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1074/jbc.M203038200","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00251083","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00219258","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1083-351X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Shigeki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Etsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nemoto, Takayuki K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takagi, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imai, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Yoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yahara, Ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayakawa, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ayuse, Takao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oi, Kumiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizuno, Akio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JBC_277_38_34959.pdf","filesize":[{"value":"479.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBC_277_38_34959.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16298/files/JBC_277_38_34959.pdf"},"version_id":"22f88c9b-3cbb-47aa-9f0b-2cc54d431c33"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Interaction between the N-terminal and middle regions is essential for the in vivo function of HSP90 molecular chaperone.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Interaction between the N-terminal and middle regions is essential for the in vivo function of HSP90 molecular chaperone."}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-09-17"},"publish_date":"2009-09-17","publish_status":"0","recid":"16298","relation_version_is_last":true,"title":["Interaction between the N-terminal and middle regions is essential for the in vivo function of HSP90 molecular chaperone."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:50:26.464697+00:00"}