{"created":"2023-05-15T16:41:39.148960+00:00","id":16353,"links":{},"metadata":{"_buckets":{"deposit":"7a304cb3-5134-4042-b89f-5d721b311f99"},"_deposit":{"created_by":2,"id":"16353","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16353"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016353","sets":["29:30"]},"author_link":["61852","61857","61856","61849","61854","61846","61845","61848","61844","61847","61851","61850","61853","61855","61843"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"863","bibliographicPageStart":"856","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"Journal of Gastroenterology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"OBJECT: The interferon-induced Jak-STAT signal alone is not sufficient to explain all the biological effects of IFN. The PI3-K pathways have emerged as a critical additional component of IFN-induced signaling. This study attempted to clarify that relationship between IFN-induced PI3-K-Akt-mTOR activity and anti-viral action. RESULT: When the human normal hepatocyte derived cell line was treated with rapamycin (rapa) before accretion of IFN-alpha, tyrosine phosphorylation of STAT-1 was diminished. Pretreatment of rapa had an inhibitory effect on the IFN-alpha-induced expression of PKR and p48 in a dose dependent manner. Rapa inhibited the IFN-alpha inducible IFN-stimulated regulatory element luciferase activity in a dose-dependent manner. However, wortmannin, LY294002 and Akt inhibitor did not influence IFN-alpha inducible luciferase activity. To examine the effect of PI3-K-Akt-mTOR on the anti-HCV action of IFN-alpha, the full-length HCV replication system, OR6 cells were used. The pretreatment of rapa attenuated its anti-HCV replication effect in comparison to IFN-alpha alone, whereas the pretreatment with PI3-K inhibitors, wortmannin and LY294002 and Akt inhibitor did not influence IFN-induced anti-HCV replication. CONCLUSION: IFN-induced mTOR activity, independent of PI3K and Akt, is the critical factor for its anti-HCV activity. Jak independent mTOR activity involved STAT-1 phosphorylation and nuclear location, and then PKR is expressed in hepatocytes.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Gastroenterology, 44(8), pp.856-863; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Japan"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19436942","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00535-009-0075-1","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c Springer 2009"},{"subitem_rights":"The original publication is available at www.springerlink.com"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09441174","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14355922","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsumoto, Azusa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichikawa, Tatsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakao, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyaaki, Hisamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Kumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimito, Masumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiyama, Motohisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miuma, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozawa, Eisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Hidetaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeshita, Shigeyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, Hironori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikeda, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Nobuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Katsumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JouGas44_856.pdf","filesize":[{"value":"923.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JouGas44_856.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16353/files/JouGas44_856.pdf"},"version_id":"b7b73fc7-3f24-4a50-8c5e-6cb79b83b1fd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"mTOR","subitem_subject_scheme":"Other"},{"subitem_subject":"STAT-1","subitem_subject_scheme":"Other"},{"subitem_subject":"Interferon","subitem_subject_scheme":"Other"},{"subitem_subject":"HCV","subitem_subject_scheme":"Other"},{"subitem_subject":"PKR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Interferon-alpha-induced mTOR activation is an anti-hepatitis C virus signal via the phosphatidylinositol 3-kinase-Akt-independent pathway.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Interferon-alpha-induced mTOR activation is an anti-hepatitis C virus signal via the phosphatidylinositol 3-kinase-Akt-independent pathway."}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-11"},"publish_date":"2010-06-11","publish_status":"0","recid":"16353","relation_version_is_last":true,"title":["Interferon-alpha-induced mTOR activation is an anti-hepatitis C virus signal via the phosphatidylinositol 3-kinase-Akt-independent pathway."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:49:04.621413+00:00"}