{"created":"2023-05-15T16:41:40.441281+00:00","id":16383,"links":{},"metadata":{"_buckets":{"deposit":"cbad37ff-9b1e-486d-a201-f2112d561692"},"_deposit":{"created_by":2,"id":"16383","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16383"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016383","sets":["8:9"]},"author_link":["62038","62042","62032","62036","62035","62030","62039","62034","62033","62040","62037","62031","62041"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"2302","bibliographicPageStart":"2299","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"Diabetologia"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"AIMS/HYPOTHESIS: We analysed the association between humoral autoreactivity to zinc transporter-8 (ZnT8) and the SLC30A8 rs13266634 polymorphism (Arg325Trp), which is located at the most distal loop in the ZnT8 protein. METHODS: Autoantibodies to ZnT8 were determined by RIA in 270 patients with type 1 diabetes using ZnT8 carboxy-terminal constructs (amino acids 268-369) carrying 325Trp(CW) and 325Arg(CR) and a hybrid construct (CW-CR). Forty-four ZnT8 autoantibody-positive sera with genomic DNA were used to examine the association between reactivity to ZnT8 constructs and the rs13266634 genotype. RESULTS: Seventy-five patients reacted to the CW-CR hybrid construct, whereas 37 and 36 patients reacted to the CW and CR constructs, respectively. All sera positive for either CW or CR autoantibodies were positive for CW-CR autoantibodies. Among 19 patients with a 325Arg(CC) genotype, 5% had CW-specific autoantibodies, 42% had CR-specific autoantibodies and 32% had dual reactivity. Conversely, 73% of 15 patients with the 325Trp(TT) genotype had CW-specific autoantibodies, no patients had CR-specific autoantibodies and 13% had dual reactivity. Nine of the ten patients (90%) with the CT genotype reacted with either CR or CW constructs. The titre of CR autoantibodies in patients carrying the C allele was significantly higher than that in TT homozygotes (p < 0.0001). In contrast, the titre of CW autoantibodies in patients carrying a T allele was significantly higher than that in CC homozygotes (p < 0.005). No evidence of an association between rs13266634 and type 1 diabetes was observed. CONCLUSIONS/INTERPRETATION: These results indicate that variant residue at amino acid 325 is a key determinant of humoral autoreactivity to ZnT8 and that the SLC30A8 genotype is an important determinant of autoantibody specificity.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Diabetologia, 51(12), pp.2299-2302; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Berlin"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18850084","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00125-008-1165-y","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c Springer-Verlag 2008"},{"subitem_rights":"The original publication is available at www.springerlink.com"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00628104","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0012186X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1432-0428","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawasaki, Eiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uga, Mho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Kazuhiko T."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuriya, Genpei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, T"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujishima, Keiichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abiru, Norio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, H"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wenzlau, Janet M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Davidson, Howard W."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hutton, J C"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, K"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"Diabetologia51_2299.pdf","filesize":[{"value":"124.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Diabetologia51_2299.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16383/files/Diabetologia51_2299.pdf"},"version_id":"80ab08d1-6a91-42fb-bcec-f3659852f9d3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Autoantibodies","subitem_subject_scheme":"Other"},{"subitem_subject":"Epitope","subitem_subject_scheme":"Other"},{"subitem_subject":"Genetics","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese population","subitem_subject_scheme":"Other"},{"subitem_subject":"SLC30A8","subitem_subject_scheme":"Other"},{"subitem_subject":"Type 1 diabetes","subitem_subject_scheme":"Other"},{"subitem_subject":"Zinc transporter-8","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Association between anti-ZnT8 autoantibody specificities and SLC30A8 Arg325Trp variant in Japanese patients with type 1 diabetes.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Association between anti-ZnT8 autoantibody specificities and SLC30A8 Arg325Trp variant in Japanese patients with type 1 diabetes."}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-20"},"publish_date":"2009-10-20","publish_status":"0","recid":"16383","relation_version_is_last":true,"title":["Association between anti-ZnT8 autoantibody specificities and SLC30A8 Arg325Trp variant in Japanese patients with type 1 diabetes."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:05:18.205413+00:00"}