{"created":"2023-05-15T16:41:41.744540+00:00","id":16413,"links":{},"metadata":{"_buckets":{"deposit":"0c11be9c-c24e-4421-9fd6-8493597e5587"},"_deposit":{"created_by":2,"id":"16413","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16413"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016413","sets":["10:11"]},"author_link":["62193","62192","62195","62194"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageEnd":"3022","bibliographicPageStart":"3015","bibliographicVolumeNumber":"312","bibliographic_titles":[{"bibliographic_title":"Experimental cell research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cell migration is regulated by focal adhesion (FA) turnover. Fibroblast growth factor-2 (FGF-2) induces FA disassembly in the murine brain capillary endothelial cell line IBE, leading to FGF-2-directed chemotaxis. We previously showed that activation of Src and Fes by FGF-2 was involved in chemotaxis of IBE cells. In this study, we examined the interplay between Src and Fes. FGF-2 treatment decreased the number of FA in IBE cells, but not in cells expressing dominant-negative Fes (denoted KE5-15 cells). FGF-2 induced the activation of Src and subsequent binding to and phosphorylation of Cas in IBE cells, but not in KE5-15 cells. Focal adhesion kinase (FAK) activation and tyrosine phosphorylation by Src were also delayed in KE5-15 cells compared to parental cells. FGF-2 induced activation of Src within FA in IBE cells, but not in KE5-15 cells. Downregulation of Fes or FAK using small interfering RNA diminished Src activation by FGF-2 within FA. These findings suggest that activation of Fes by FGF-2 enhances FAK-dependent activation of Src within FA, promoting FGF-2-induced disassembly of focal adhesions.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Experimental cell research, 312(16), pp.3015-3022; 2006","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"16884713","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.yexcr.2006.06.032","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2006 Published by Elsevier Inc."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00641011","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00144827","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1090-2422","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kanda, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyata, Yasuyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanetake, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Smithgall, Thomas E"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"ECR312_3015.pdf","filesize":[{"value":"413.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ECR312_3015.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16413/files/ECR312_3015.pdf"},"version_id":"9afab244-e7ed-40c7-87ae-f70473f809f6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"FGF-2","subitem_subject_scheme":"Other"},{"subitem_subject":"Src","subitem_subject_scheme":"Other"},{"subitem_subject":"Fes","subitem_subject_scheme":"Other"},{"subitem_subject":"FAK","subitem_subject_scheme":"Other"},{"subitem_subject":"Endothelial cell","subitem_subject_scheme":"Other"},{"subitem_subject":"Focal adhesion","subitem_subject_scheme":"Other"},{"subitem_subject":"Migration","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fibroblast growth factor-2 induces the activation of Src through Fes, which regulates focal adhesion disassembly.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fibroblast growth factor-2 induces the activation of Src through Fes, which regulates focal adhesion disassembly."}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-27"},"publish_date":"2009-10-27","publish_status":"0","recid":"16413","relation_version_is_last":true,"title":["Fibroblast growth factor-2 induces the activation of Src through Fes, which regulates focal adhesion disassembly."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:04:40.754294+00:00"}