{"created":"2023-05-15T16:41:43.794154+00:00","id":16461,"links":{},"metadata":{"_buckets":{"deposit":"2f3ce6f6-fe32-4e5f-bbc9-7c293787d884"},"_deposit":{"created_by":2,"id":"16461","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16461"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016461","sets":["8:9"]},"author_link":["62528","62529","62524","62525","62532","62523","62522","62527","62526","62530","62531"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"385","bibliographicPageStart":"379","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"Molecular Medicine Reports"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Oxidative stress is known to play a key role in the progression of liver disease, including non-alcoholic steatohepatitis (NASH), which is often accompanied by hyperglycemia. This study examined the influence of high glucose on oxidative stress-induced hepatic cell death. Hc cells, a normal human hepatocyte-derived cell line, were cultured in normal-to-high glucose (5.5-22 mM)-containing medium with varying concentrations (0.01-1 mM) of hydrogen peroxide. In certain experiments, cyclosporine A (CyA), which inhibits the mitochondrial permeability transition (MPT) pore, or Z-VAD-FMK (z-VAD), a pan-caspase inhibitor, were added to the medium. Cell viability was evaluated using a colorimetric assay. The mode of cell death was determined by nuclear staining methods using Hoechst 33258 and Sytox green. Neither high glucose (22 mM) nor 0.05-0.5 mM of hydrogen peroxide alone killed Hc cells. However, a combination of the two induced cell death, causing the nuclei of Hc cells to become expanded rather than condensed, and the nuclear membrane to become weak. CyA, but not z-VAD, blocked cell death. These results suggest that a high glucose condition may cause human hepatocytes to undergo hydrogen peroxide-induced necrotic cell death.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Molecular Medicine Reports, 1(3), pp.379-385; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Spandidos Publications"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17912997","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1791-3004","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shibata, Hidetaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichikawa, Tatsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakao, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyaaki, Hisamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeshita, Shigeyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akiyama, Motohisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimoto, Masumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miuma, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Shougo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamasaki, Hironori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Katsumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"MMR_1_3_379.pdf","filesize":[{"value":"202.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MMR_1_3_379.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16461/files/MMR_1_3_379.pdf"},"version_id":"72ffbe48-6036-48a2-9502-0a11f1f0eebe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"high glucose","subitem_subject_scheme":"Other"},{"subitem_subject":"reactive oxygen species","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocyte cell death","subitem_subject_scheme":"Other"},{"subitem_subject":"necrosis","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclosporine A","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A high glucose condition sensitizes human hepatocytes to hydrogen peroxide-induced cell death","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A high glucose condition sensitizes human hepatocytes to hydrogen peroxide-induced cell death"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-09"},"publish_date":"2009-11-09","publish_status":"0","recid":"16461","relation_version_is_last":true,"title":["A high glucose condition sensitizes human hepatocytes to hydrogen peroxide-induced cell death"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:46:20.497539+00:00"}