@article{oai:nagasaki-u.repo.nii.ac.jp:00016507,
 author = {Takaoka, Hiroyuki and Suzuki, Hiroshi and Noda, Shin-ichi and Tada, Isao and Sara, Rodulfo and Lucena, Pachano and Jacinto, Convit},
 issue = {2},
 journal = {日本熱帯医学会雑誌, Japanese journal of tropical medicine and hygiene},
 month = {Jun},
 note = {ベネズエラ北部のオンコセルカ症伝搬プユ種であるSimulium metallicumのOnchocerca volvulusに対する感受性の程度を実験感染により検討した。実験lこは，O. volvulusミクロフィラリア保有者の脚から充分吸血した野外の雌成虫を用いた。これらの吸血個体を22-280℃ の温度条件下で個別に飼育し，毎日死亡した個体を解剖することにより幼虫の発育を調べた。その結果，吸血後5日と6日の聞に死亡したプユから最初の第III期幼虫が見いだされた。幼虫発育は不揃いであったが異常形の幼虫は見られなかった。ブユ体内で見いだされた幼虫のうち第III期幼虫の占める割合は吸血後6-8日に剖検したプユ体内で6%であったが， 9-11日に調べたブユでは69%と日数の経過とともに増加した。同期聞におけるブユの頭部に見いだされた第III期幼虫の割合も25%から54%へ上昇した。すなわち，S. metallicum体内でのO.volvulus幼虫の発育は不揃いであるが，ブユが長く生存すれば，とりこまれたミクロフィラリアの多くは第III期幼虫まで発育し，人へ感染する機会が多いことが示された。これらの結果と既に報告されているグアテマラとコロンピアでの実験感染の結果を比較すると，ベネズエラのS.metallicumは上記2国の同一ブユ種より，O. volvulusに対して高い感受性をもっていることが示唆された。, The degree of susceptibility of Simulium metallicum to infection with Onchocerca volvulus was studied in Venezuela. Wild flies which had fed on the infected volunteer were maintained at a temperature varying between 22°C and 28°C, and all flies dying every 24 hours were dissected for larval development. Third-stage larvae were first seen in the head of flies dying between five and six days. Few abnormal and deformed larvae were observed. The larval development was asynchronous; only 41 of 117 larvae recovered from 32 flies examined on days 6-11 were in the third stage, the remaining larvae being still in earlier stages. However, the proportion of third-stage larvae among all larvae recovered increased from 6 to 69 per cent, with the passage of time from days 6-8 to 9-11. Likewise, the percentage of third-stage larvae found in the head during the same period rose from 25 to 54 per cent. These results indicate that despite the asynchronous and retarded development, many larvae can develop to the infective stage in S. metallicllm and the possibility of infective larvae capable of being eventually inoculated into man is high, when flies could survive more. It is suggested that the susceptibility of S. metallicum in Venezuela to the indigenous strain of O. volvulus is relatively high, as compared with that of the same species in Guatemala or Colombia., 日本熱帯医学会雑誌, vol.12(2), pp.89-96; 1984},
 pages = {89--96},
 title = {SUSCEPTIBILITY OF SIMULIUM METALLICUM TO INFECTION WITH ONCHOCERCA VOLVULUS IN VENEZUELA},
 volume = {12},
 year = {1984}
}