{"created":"2023-05-15T16:41:51.187594+00:00","id":16633,"links":{},"metadata":{"_buckets":{"deposit":"e1238c91-13d2-4c36-bf63-7976e77cbda6"},"_deposit":{"created_by":2,"id":"16633","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16633"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016633","sets":["35:36"]},"author_link":["63774","63773","63775","63776","63777","63772"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Pt 1","bibliographicPageEnd":"305","bibliographicPageStart":"297","bibliographicVolumeNumber":"89","bibliographic_titles":[{"bibliographic_title":"The Journal of general virology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A Mus dunni tail fibroblast (MDTF) cell line is highly resistant to infection by ecotropic Moloney murine leukemia virus (Mo-MLV). The cationic amino acid transporter type 1 (CAT1) paralogues of murine NIH 3T3 and MDTF cells (mCAT1 and dCAT1, respectively) contain two conserved N-linked glycosylation sites in the third extracellular loop (ECL3, the putative Mo-MLV binding site). Glycosylation of dCAT1 inhibits Mo-MLV infection, but that of mCAT1 does not. Compared with mCAT1, dCAT1 possesses an Ile-to-Val substitution at position 214 and a Gly insertion at position 236 in the ECL3. To determine the residues responsible for the loss of dCAT1 receptor function, mutants of mCAT1 were constructed. The mCAT1/insG receptor (with a Gly residue inserted at mCAT1 position 236) had greatly reduced Mo-MLV receptor function compared with mCAT1. Treatment of mCAT1/insG-expressing cells with tunicamycin, an N-linked glycosylation inhibitor, increased the transduction titre. In addition, the reduced susceptibility to Mo-MLV observed with mCAT1/insG-expressing cells correlated with impaired binding of Mo-MLV. These results show that a single amino acid insertion confers mCAT1 receptor properties on dCAT1 and provide an important insight into the co-evolution of virus-host interactions.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"The Journal of general virology, 89(1), pp.297-305; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for General Microbiology"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18089754","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1099/vir.0.83430-0","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2008 SGM."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00698722","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00221317","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1465-2099","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshii, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamiyama, Haruka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amanuma, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oishi, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Naoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kubo, Yoshinao"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JGV89_297.pdf","filesize":[{"value":"594.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JGV89_297.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16633/files/JGV89_297.pdf"},"version_id":"68a7bda7-5b24-4d02-a101-df38b920f001"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mechanisms underlying glycosylation-mediated loss of ecotropic receptor function in murine MDTF cells and implications for receptor evolution.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanisms underlying glycosylation-mediated loss of ecotropic receptor function in murine MDTF cells and implications for receptor evolution."}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-27"},"publish_date":"2009-11-27","publish_status":"0","recid":"16633","relation_version_is_last":true,"title":["Mechanisms underlying glycosylation-mediated loss of ecotropic receptor function in murine MDTF cells and implications for receptor evolution."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:02:25.189427+00:00"}