@article{oai:nagasaki-u.repo.nii.ac.jp:00016648,
 author = {Motoshima, Maiko and Yanagihara, Katsunori and Yamamoto, Kazuko and Morinaga, Yoshitomo and Matsuda, Junichi and Sugahara, Kazuyuki and Hirakata, Yoichi and Yamada, Yasuaki and Kohno, Shigeru and Kamihira, Shimeru},
 issue = {2},
 journal = {Diagnostic microbiology and infectious disease},
 month = {Jun},
 note = {In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase., Diagnostic microbiology and infectious disease, 61(2), pp.222-226; 2008},
 pages = {222--226},
 title = {Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.},
 volume = {61},
 year = {2008}
}