{"created":"2023-05-15T16:41:56.717879+00:00","id":16759,"links":{},"metadata":{"_buckets":{"deposit":"08666343-5778-44e2-942f-cb26c92dfb1c"},"_deposit":{"created_by":2,"id":"16759","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16759"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016759","sets":["29:30"]},"author_link":["64710","64716","64711","64718","64720","64713","64719","64712","64717","64709","64715","64714"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-10-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"e7655","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"PloS one"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: Intracellular proteases constitute a class of promising drug discovery targets. Methods for high throughput screening against these targets are generally limited to in vitro biochemical assays that can suffer many technical limitations, as well as failing to capture the biological context of proteases within the cellular pathways that lead to their activation. METHODS #ENTITYSTARTX00026; FINDINGS: We describe here a versatile system for reconstituting protease activation networks in yeast and assaying the activity of these pathways using a cleavable transcription factor substrate in conjunction with reporter gene read-outs. The utility of these versatile assay components and their application for screening strategies was validated for all ten human Caspases, a family of intracellular proteases involved in cell death and inflammation, including implementation of assays for high throughput screening (HTS) of chemical libraries and functional screening of cDNA libraries. The versatility of the technology was also demonstrated for human autophagins, cysteine proteases involved in autophagy. CONCLUSIONS: Altogether, the yeast-based systems described here for monitoring activity of ectopically expressed mammalian proteases provide a fascile platform for functional genomics and chemical library screening.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PloS one, 4(10), e7655; 2009","subitem_description_type":"Other"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"19876397","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0007655","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2009 Hayashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayashi, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cuddy, Michael"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shu, Vincent Chih-Wen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yip, Kenneth W"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Madiraju, Charitha"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Diaz, Paul"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuyama, Toshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaibara, Muneshige"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniyama, Kohtaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vasile, Stefan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sergienko, Eduard"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Reed, John C"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"PLoS4_e7655.pdf","filesize":[{"value":"766.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS4_e7655.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16759/files/PLoS4_e7655.pdf"},"version_id":"8c7ea4da-f879-4c2d-ab81-f84ab694a3f0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Versatile assays for high throughput screening for activators or inhibitors of intracellular proteases and their cellular regulators.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Versatile assays for high throughput screening for activators or inhibitors of intracellular proteases and their cellular regulators."}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-15"},"publish_date":"2009-12-15","publish_status":"0","recid":"16759","relation_version_is_last":true,"title":["Versatile assays for high throughput screening for activators or inhibitors of intracellular proteases and their cellular regulators."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:37:48.495981+00:00"}