{"created":"2023-05-15T16:41:56.846038+00:00","id":16762,"links":{},"metadata":{"_buckets":{"deposit":"91e7b994-2d8b-45b7-8083-252b66074dfd"},"_deposit":{"created_by":2,"id":"16762","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16762"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016762","sets":["27:28"]},"author_link":["64741","64740","64742","64743","64744"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"946","bibliographicPageStart":"940","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"International endodontic journal"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"AIM: To examine the effect of carbon dioxide laser irradiation on mineralization in dental pulp cells. METHODOLOGY: Rat dental pulp cells were irradiated with a carbon dioxide laser at 2 W output power for 20, 40 and 60 s, and were cultured in ascorbic acid and beta-glycerophosphate containing media. Cell viability was examined 24 h after laser irradiation by a modified MTT assay. Alizarin Red S staining was performed 10 days after laser irradiation. The amounts of secreted collagen from the cells after irradiation were quantified following Sirius Red staining. The expression levels of collagen type I and HSP47, collagen-binding stress protein, were analysed by real-time PCR. HSP47 protein expression was examined by Western blotting. Statistical analysis was performed using one-way analysis of variance (anova) followed by the Tukey's multiple comparison test. RESULTS: The cell viability was not affected by laser irradiation at 2 W for up to 40 s. However, it was significantly decreased by 20% at 60 s (P < 0.05). The amount of mineralization after 10 days of irradiation at 2 W for 40 s was significantly increased in comparison to the other conditions (P < 0.05). The extracellular collagen production was significantly increased by 73% on day 2 and 38% on day 4 after laser irradiation (P < 0.05). Although collagen type I gene expression was not changed by laser irradiation, HSP47 gene and protein expression was induced within 12 and 24 h, respectively. CONCLUSIONS: These results suggested that carbon dioxide laser irradiation stimulated mineralization in dental pulp cells. The laser irradiation also increased HSP47 expression but not collagen gene expression.","subitem_description_type":"Abstract"}]},"item_2_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The definitive version is available at www.blackwell-synergy.com","subitem_description_type":"Other"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International endodontic journal, 42(10), pp.940-946; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Blackwell Publishing Inc."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19751293","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/j.1365-2591.2009.01598.x","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2009 International Endodontic Journal"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00233864","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01432885","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13652591","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasuda, Y"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohtomo, E"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukuba, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, T"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"IEJ42_940.pdf","filesize":[{"value":"477.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IEJ42_940.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16762/files/IEJ42_940.pdf"},"version_id":"b25db17f-a392-48bc-a273-71183b36a484"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Carbon dioxide laser","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen","subitem_subject_scheme":"Other"},{"subitem_subject":"Dental pulp cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Heat shock protein 47","subitem_subject_scheme":"Other"},{"subitem_subject":"Mineralization","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells."}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-05-06"},"publish_date":"2010-05-06","publish_status":"0","recid":"16762","relation_version_is_last":true,"title":["Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T19:09:46.265359+00:00"}