@article{oai:nagasaki-u.repo.nii.ac.jp:00016786,
 author = {Kurosaki, Tomoaki and Kitahara, Takashi and Kawakami, Shigeru and Higuchi, Yuriko and Yamaguchi, Ayumi and Nakagawa, Hiroo and Kodama, Yukinobu and Hamamoto, Tomoyuki and Hashida, Mitsuru and Sasaki, Hitoshi},
 issue = {3},
 journal = {Journal of controlled release : official journal of the Controlled Release Society},
 month = {Mar},
 note = {In the present study, we developed some novel gene delivery vectors, coated cationic complexes with gamma-polyglutamic acid (gamma-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the gamma-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These gamma-PGA-coated complexes had high gene expression without cytotoxicity and toxicities to the erythrocytes. In in vivo transfection experiments, polyplexes showed high transfection efficiency over 10(5) RLU/g in the lung tissue after intravenous injection, although gamma-PGA-coated polyplexes showed a high value in the spleen. High transfection efficiency in lipoplexes and gamma-PGA-coated lipoplexes was observed in the spleen and lung. Thus, gamma-PGA-coated vectors are useful for clinical gene therapy., Journal of controlled release, 142(3), pp.404-410; 2010},
 pages = {404--410},
 title = {γ-Polyglutamic acid-coated vectors for effective and safe gene therapy.},
 volume = {142},
 year = {2010}
}