{"created":"2023-05-15T16:41:57.863574+00:00","id":16786,"links":{},"metadata":{"_buckets":{"deposit":"91e48e38-2c41-48dc-ac45-f1ba602dfe07"},"_deposit":{"created_by":2,"id":"16786","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16786"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016786","sets":["10:11"]},"author_link":["64900","64899","64902","64905","64906","64901","64907","64898","64904","64903"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"410","bibliographicPageStart":"404","bibliographicVolumeNumber":"142","bibliographic_titles":[{"bibliographic_title":"Journal of controlled release : official journal of the Controlled Release Society"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, we developed some novel gene delivery vectors, coated cationic complexes with gamma-polyglutamic acid (gamma-PGA) for effective and safe gene therapy. Cationic complexes were constructed with pDNA and cationic vectors, such as poly-L-arginine hydrochloride (PLA), poly-L-lysine hydrobromide (PLL), N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol (Chol) liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. The cationic complexes showed high gene expression with strong cytotoxicity in melanoma B16-F10 cells. The cationic complexes were also strongly toxic to erythrocytes. On the other hand, the gamma-PGA was able to coat all cationic complexes and form stable nano-sized particles with negative charges. These gamma-PGA-coated complexes had high gene expression without cytotoxicity and toxicities to the erythrocytes. In in vivo transfection experiments, polyplexes showed high transfection efficiency over 10(5) RLU/g in the lung tissue after intravenous injection, although gamma-PGA-coated polyplexes showed a high value in the spleen. High transfection efficiency in lipoplexes and gamma-PGA-coated lipoplexes was observed in the spleen and lung. Thus, gamma-PGA-coated vectors are useful for clinical gene therapy.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of controlled release, 142(3), pp.404-410; 2010","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19931327","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jconrel.2009.11.010","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2009 Elsevier B.V. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10458678","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01683659","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1873-4995","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurosaki, Tomoaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitahara, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Yuriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Ayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamamoto, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"JCR142_404.pdf","filesize":[{"value":"624.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JCR142_404.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16786/files/JCR142_404.pdf"},"version_id":"5acfaa43-f6ed-4363-9d6a-cd6bdb4460b4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"γ-Polyglutamic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Cationic liposome","subitem_subject_scheme":"Other"},{"subitem_subject":"Cationic polymer","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-viral gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Ternary complex","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"γ-Polyglutamic acid-coated vectors for effective and safe gene therapy.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"γ-Polyglutamic acid-coated vectors for effective and safe gene therapy."}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-01-05"},"publish_date":"2010-01-05","publish_status":"0","recid":"16786","relation_version_is_last":true,"title":["γ-Polyglutamic acid-coated vectors for effective and safe gene therapy."],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T18:58:30.327392+00:00"}