{"created":"2023-05-15T16:41:59.684797+00:00","id":16829,"links":{},"metadata":{"_buckets":{"deposit":"20707db6-b9e1-48fe-a839-662da42c3973"},"_deposit":{"created_by":2,"id":"16829","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"16829"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00016829","sets":["29:30"]},"author_link":["65168","65164","65167","65166","65165","65169","65163"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"542","bibliographicPageStart":"533","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Antiviral therapy"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: This study was conducted to construct a basis for a therapeutic strategy against human T-lymphotropic virus type-I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) using a compound that contained a disulfide moiety, prosultiamine, which is a homologue of allithiamine originally synthesized by allicin and thiamine-thiol, for the targeting of HTLV-I-infected cells. METHODS: First, we analysed the apoptotic pathway in allicin or prosultiamine treatment against an HTLV-I-infected T-cell line (HCT-1), derived from an HAM/TSP patient, by flow cytometry and western blot. Second, we evaluated the effect of targeting HTLV-I-infected cells in a prosultiamine in vitro treatment and in a clinical trial in HAM/TSP patients by quantitative PCR analysis of HTLV-I proviral load. RESULTS: Prosultiamine, like allicin, induced caspase-dependent apoptosis against HCT-1 cells. The fact that the loss of mitochondrial membrane potential was recovered in z-VAD-fmk-pretreated HCT-1 cells with prosultiamine treatment suggested that prosultiamine can induce caspase-dependent apoptosis through the mitochondrial pathway. On the basis of data showing that prosultiamine in vitro treatment against peripheral blood CD4(+) T-cells of HAM/TSP patients induced a significant decrease of HTLV-I proviral copy numbers by apoptosis of HTLV-I-infected cells, we treated six HAM/TSP patients with intravenous administration of prosultiamine for 14 days. As a result of this treatment, the copy numbers of HTLV-I provirus in peripheral blood decreased to approximately 30-50% of their pretreatment levels with some clinical benefits in all patients. CONCLUSIONS: Our results suggest that prosultiamine has the potential to be a new therapeutic tool that targets HTLV-I-infected cells in HAM/TSP.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Antiviral therapy, 14(4), pp.533-542; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Medical Press"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19578238","subitem_relation_type_select":"PMID"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2009 International Medical Press."},{"subitem_rights":"This is the author’s version of a work accepted for publication by International Medical Press. Changes resulting from the publishing process, including peer review, editing and formatting, might not be reflected in this document. A definitive version was published in Antiviral Therapy, (Vol No14, Issue No4), 2009, c2009 International Medical Press."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11642446","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13596535","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nishiura, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tatsufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukushima, Naomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ida, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aramaki, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Katsumi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"AntThe14_533.pdf","filesize":[{"value":"822.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AntThe14_533.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/16829/files/AntThe14_533.pdf"},"version_id":"79f658a3-8f2a-4b01-bba4-1fe5df610227"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HTLV-I","subitem_subject_scheme":"Other"},{"subitem_subject":"HAM/TSP","subitem_subject_scheme":"Other"},{"subitem_subject":"Treatment","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Disulfide moiety","subitem_subject_scheme":"Other"},{"subitem_subject":"Prosultiamine","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Disulfide-mediated apoptosis of human T-lymphotrophc virus type-I (HTLV-I)-infected cells in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Disulfide-mediated apoptosis of human T-lymphotrophc virus type-I (HTLV-I)-infected cells in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis."}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-01-19"},"publish_date":"2010-01-19","publish_status":"0","recid":"16829","relation_version_is_last":true,"title":["Disulfide-mediated apoptosis of human T-lymphotrophc virus type-I (HTLV-I)-infected cells in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-17T20:36:04.819901+00:00"}