{"created":"2023-05-15T16:42:36.578506+00:00","id":17683,"links":{},"metadata":{"_buckets":{"deposit":"d9b8bff7-80b5-4577-90cc-def171af2607"},"_deposit":{"created_by":2,"id":"17683","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"17683"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00017683","sets":["50:51"]},"author_link":["69893","69889","69892","69885","69890","69887","69888","69891","69895","69886","69894","69884"],"item_2_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Limitted Degradation of α-actinin in the Ordinary Muscle of Cultured Red Sea Bream during Storage in Ice"}]},"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"727","bibliographicPageStart":"723","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"日本水産学会誌"},{"bibliographic_title":"Bulletin of the Japanese Society of Scientific Fisheries","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"養殖マダイ普通筋α-アクチニン抗体を作成し, これを用いて養殖マダイ氷蔵中における普通筋中α-アクチニンの分解をSDS-PAGEとイムノブロッティングで解析した。作成したα-アクチニン抗体はマダイ普通筋中のα-アクチニンのみならず, そのパパイン分解物とも反応性を示した。本抗体は免疫電顕による観察で, 筋原線維のZ線に特異的に反応し, 他の細胞内小器官等には反応を認めなかった。普通筋中α-アクチニンは氷蔵1日目から自己消化され, 5日目以降ではすべて分子量90kDa∿100kDaの成分に限定分解されていた。","subitem_description_type":"Abstract"},{"subitem_description":"Anti-α-actinin IgG was prepared from rat serum immunized with purified α-actinin in the ordinary muscle of red sea bream. Anti-α-actinin IgG showed immune reactivity against not only α-actinin in ordinary muscle but also papain-digests of the α-actinin. According to immunoelectron microscopic method, α-actinin was detected on Z line of ordinary muscle cells and non-specific reactions were not detected on another organelles. The changes of α-actinin in the ordinary muscle of cultured red sea bream during storage in ice were examined by SDS-PAGE followed by its immunoblotting. It was found that the limited digestion of α-actinin in ordinary muscle of cultured red sea bream started on day one in ice, then, native α-actinin was not detected on day 5 and 7. These results suggested that the weakening of Z-disks in ordinary muscle was caused by the disappearance of α-actinin and resulted in the softness of the flesh texture of cultured red sea bream.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries. 2001, 67(4), p.723-727","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"69890","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tachibana, Katsuyasu"}]},{"nameIdentifiers":[{"nameIdentifier":"69891","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Suzuki, Hidekazu"}]},{"nameIdentifiers":[{"nameIdentifier":"69892","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yagi, Motoaki"}]},{"nameIdentifiers":[{"nameIdentifier":"69893","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Mishima, Tosio"}]},{"nameIdentifiers":[{"nameIdentifier":"69894","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hara, Kenji"}]},{"nameIdentifiers":[{"nameIdentifier":"69895","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tsuchimoto, Mutsuyosi"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"社団法人日本水産学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"社団法人日本水産学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00193422","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00215392","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"橘, 勝康"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 秀一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"八木, 基明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三嶋, 敏雄"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"原, 研治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"槌本, 六良"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"suisan67_4_723_727.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"suisan67_4_723_727.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/17683/files/suisan67_4_723_727.pdf"},"version_id":"fcd9ef86-a283-4252-b97d-f7af49b2d351"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"α-アクチニン","subitem_subject_scheme":"Other"},{"subitem_subject":"養殖マダイ","subitem_subject_scheme":"Other"},{"subitem_subject":"イムノブロッティング","subitem_subject_scheme":"Other"},{"subitem_subject":"免疫電子顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"Z線","subitem_subject_scheme":"Other"},{"subitem_subject":"筋原線維","subitem_subject_scheme":"Other"},{"subitem_subject":"SDS-PAGE","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解"}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-04-14"},"publish_date":"2009-04-14","publish_status":"0","recid":"17683","relation_version_is_last":true,"title":["養殖マダイの氷蔵中における普通筋中 α-アクチニンの限定分解"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:27:30.455188+00:00"}