{"created":"2023-05-15T16:42:40.234536+00:00","id":17766,"links":{},"metadata":{"_buckets":{"deposit":"0287a09b-cfa5-464b-8dcf-ee7ecfa58121"},"_deposit":{"created_by":2,"id":"17766","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"17766"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00017766","sets":["27:28"]},"author_link":["70218","70215","70217","70214","70216"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"285","bibliographicPageStart":"277","bibliographicVolumeNumber":"390","bibliographic_titles":[{"bibliographic_title":"Biological chemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus warneri secrete glutamyl endopeptidases, designated GluV8, GluSE and GluSW, respectively. The order of their protease activities was GluSE<GluSW<<GluV8. The present study investigated the mechanism that causes these differences. Expression of chimeric proteins between GluV8 and GluSE revealed that the difference was primarily attributed to amino acids at residues 170-195, which defined the intrinsic protease activity, and additionally to residues 119-169, which affected the proteolysis sensitivity. Among nine substitutions present in residues 170-195 of the three proteases, the substitutions at positions 185, 188 and 189 were responsible for the changes in their activities; and the combination of W185, V188 and P189, which naturally occurred on GluV8, exerted the highest protease activity. Among them, W185 and P189 were indispensable for the full activity; but V188 could be replaced by hydrophobic amino acids. These three amino acid residues appeared to create a substrate-binding pocket together with the catalytic triad and the N-terminal V1, and therefore, defined the K(m) values of the proteases. This study also describes the way to produce a chimeric form of GluSE and GluV8 that was resistant to proteolysis, and therefore, possessed activity 4-fold higher than that of the wild-type recombinant GluV8.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological chemistry, 390(3), pp.277-285; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Walter de Gruyter GmbH & Co. KG"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"19090719","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1515/BC.2009.027","subitem_relation_type_select":"DOI"}}]},"item_2_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"www.degruyter.com/journals/bc"}]}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Walter de Gruyter."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11099140","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14316730","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14374315","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nemoto, Takayuki K"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimoyama, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Shigenobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohara-Nemoto, Yuko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"BioChem390_277.pdf","filesize":[{"value":"603.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BioChem390_277.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/17766/files/BioChem390_277.pdf"},"version_id":"85e02aec-67ce-4fda-8b2b-8133992e8761"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Glutamyl endopeptidase","subitem_subject_scheme":"Other"},{"subitem_subject":"Staphylococcus aureus","subitem_subject_scheme":"Other"},{"subitem_subject":"Staphylococcus epidermidis","subitem_subject_scheme":"Other"},{"subitem_subject":"Substrate-binding pocket","subitem_subject_scheme":"Other"},{"subitem_subject":"V8 protease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases."}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-11"},"publish_date":"2010-06-11","publish_status":"0","recid":"17766","relation_version_is_last":true,"title":["Determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:23:44.008713+00:00"}