@article{oai:nagasaki-u.repo.nii.ac.jp:00017983,
 author = {Mitsui, Yoshinori and Tanimori, Hideaki and Kitagawa, Tsunehiro and Fujimaki, Yasunori and Aoki, Yoshiki},
 issue = {3},
 journal = {American Journal of Tropical Medicine and Hygiene},
 month = {Mar},
 note = {A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3?10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 μg/kg of body weight., American Journal of Tropical Medicine and Hygiene, 54(3), pp.243-248; 1996},
 pages = {243--248},
 title = {Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin},
 volume = {54},
 year = {1996}
}