{"created":"2023-05-15T16:42:50.658868+00:00","id":17983,"links":{},"metadata":{"_buckets":{"deposit":"afa21be4-a68d-4f0d-87d2-d02d515ae696"},"_deposit":{"created_by":2,"id":"17983","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"17983"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00017983","sets":["35:36"]},"author_link":["71287","71286","71285","71284","71288"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"248","bibliographicPageStart":"243","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"American Journal of Tropical Medicine and Hygiene"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3?10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 μg/kg of body weight.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"American Journal of Tropical Medicine and Hygiene, 54(3), pp.243-248; 1996","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society of Tropical Medicine and Hygiene"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"8600758","subitem_relation_type_select":"PMID"}}]},"item_2_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.ajtmh.org/cgi/content/abstract/54/3/243"}]}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 1996 by The American Society of Tropical Medicine and Hygiene"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00521439","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00029637","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mitsui, Yoshinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanimori, Hideaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitagawa, Tsunehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimaki, Yasunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoki, Yoshiki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"ajtmh54_243.pdf","filesize":[{"value":"537.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ajtmh54_243.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/17983/files/ajtmh54_243.pdf"},"version_id":"72025bea-c1c0-4400-ba24-7d72961e4422"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-05-13"},"publish_date":"2009-05-13","publish_status":"0","recid":"17983","relation_version_is_last":true,"title":["Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:14:38.228387+00:00"}