{"created":"2023-05-15T16:42:52.275927+00:00","id":18008,"links":{},"metadata":{"_buckets":{"deposit":"49c429a5-e029-420d-8c9b-9dc5e59b690a"},"_deposit":{"created_by":2,"id":"18008","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"18008"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00018008","sets":["10:11"]},"author_link":["71491","71494","71493","71488","71495","71498","71487","71496","71490","71497","71492","71489"],"item_2_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Flow Injection Analysis with Luminol Chemiluminescence Detection for Evaluation of Quenching Effects of Grape Seed Extracts and Polyphenolics against Reactive Oxygen Species"}]},"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-12-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"936","bibliographicPageStart":"931","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"分析化学"},{"bibliographic_title":"BUNSEKI KAGAKU","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"フローインジェクション分析(FIA)-ルミノール化学発光検出による簡便・迅速な活性酸素種(ROS)に対する消去能の評価法を開発し,これを市販のブドウ種子抽出物及びそれらに含まれることが知られているポリフェノール類に適用した.ヒドロキシラジカル(^・OH),一重項酸素(^1O_2),パーオキシナイトライトCONOO^-)及びスーパーオキシドアニオン(O_2^-)等のROSとルミノールとの反応によって生じる化学発光に対して,抗酸化物質含有試料の添加による減少率をROS消去能とした.本FIAシステムは,1検体を1分以内に測定可能であり,各ROSにおける,抗酸化物質含有試料を未添加時のブランク発光の精度も良好であった(相対標準偏差?4.5%, n=5).用量-反応曲線から算出したシアニジンのEC_<50>値(n=3)はそれぞれ50±4ng/assay (^・OH), 148±8(^1O_2), 36±4(ONOO^-)及び95±8(O_2^-)であった.カルコンを除くペラルゴニジン,デルフィニジン及びレスベラトロール等のポリフェノール類もROSに対する強い消去能を示した.また,ブドウ種子抽出物でも同様なROS消去能が観察された.","subitem_description_type":"Abstract"},{"subitem_description":"A simple and rapid FIA method for measurement of quenching effects of grape seed extracts and polyphenolics against reactive oxygen species (ROS) was developed. This method is based on the determination of luminol chemiluminescence generated with ROS such as hydroxyl radical (^・OH), singlet oxygen (^1O_2), peroxynitrite (ONOO^-) and superoxide anion (O_2^-). More than 1 sample/min could be measured with satisfactory precision. The total reagent amount in this method could be reduced to one tenth of that for our previous batch method. The grape seed extracts A and B were successfully evaluated by the proposed method. The EC_<50> of cyanidin as a representative polyphenolic against ^・OH, ^1O_2, ONOO^・ and O_2^- were 50±4, 148±8, 36±4, and 95±8ng/assay (n=3), respectively. Except for chalcone, the other polyphenolics such as pelargonidin, delphinidin and trans-resveratrol also showed the high quenching effects against ROS.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"分析化学, 55(12), pp. 931-936; 2006","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"71493","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Wada, Mitsuhiro"}]},{"nameIdentifiers":[{"nameIdentifier":"71494","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Katoh, Masayuki"}]},{"nameIdentifiers":[{"nameIdentifier":"71495","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kido, Hirotsugu"}]},{"nameIdentifiers":[{"nameIdentifier":"71496","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakashima, Mihoko  N."}]},{"nameIdentifiers":[{"nameIdentifier":"71497","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Kuroda, Naotaka"}]},{"nameIdentifiers":[{"nameIdentifier":"71498","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakashima, Kenichiro"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2116/bunsekikagaku.55.931","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The Japan Society for Analytical Chemistry (c) 2006"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00222633","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"05251931","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"和田, 光弘"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤, 正之"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"城戸, 浩胤"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中嶋, 弥穂子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒田, 直敬"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中島, 憲一郎"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"BunsekiKagaku55_931.pdf","filesize":[{"value":"357.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BunsekiKagaku55_931.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/18008/files/BunsekiKagaku55_931.pdf"},"version_id":"6ecb7927-b829-4da9-b151-d4b9c6be65f3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"flow injection analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"chemiluminescent assay","subitem_subject_scheme":"Other"},{"subitem_subject":"grape seed extract","subitem_subject_scheme":"Other"},{"subitem_subject":"polyphenolics","subitem_subject_scheme":"Other"},{"subitem_subject":"reactive oxygen species","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-05-18"},"publish_date":"2009-05-18","publish_status":"0","recid":"18008","relation_version_is_last":true,"title":["フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:13:36.099413+00:00"}