{"created":"2023-05-15T16:43:04.111867+00:00","id":18268,"links":{},"metadata":{"_buckets":{"deposit":"e08f9ead-16c2-4053-8121-f7ca5b72141d"},"_deposit":{"created_by":2,"id":"18268","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"18268"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00018268","sets":["10:11"]},"author_link":["72906","72909","72913","72911","72910","72907","72912","72908"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"2219","bibliographicPageStart":"2210","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Human Reproduction"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: We investigated the role of human heat-shock protein 70 (Hsp70) in Toll-like receptor 4 (TLR4)-mediated growth of endometriosis. METHODS: TLR4 expression was examined in macrophages (Mφ) isolated in primary culture from the peritoneal fluid of women with and without endometriosis. The production of a number of macromolecules by non-treated Mφ, Hsp70-treated Mφ and after treatment with anti-TLR4 antibody was examined by enzyme linked immunosorbent assay (ELISA). The single and combined effects of Hsp70 and lipopolysaccharide (LPS) on the growth of endometrial stromal cells were analyzed by 5-bromo-2-deoxyuridine (BrdU) incorporation study. Hsp70 levels in eutopic and ectopic endometria were measured by ELISA. RESULTS: TLR4 was detected in isolated Mφ at protein and gene level. Hsp70 (10 μg/ml) significantly stimulated the production of hepatocyte growth factor, vascular endothelial cell growth factor, interleukin-6 and tumor necrosis factor alpha by Mφ derived from women with endometriosis compared with Mφ derived from women with no endometriosis (P < 0.05 for each). This effect of Hsp70 was abrogated after pretreatment of Mφ with anti-TLR4 antibody. BrdU incorporation indicated that Hsp70 significantly enhanced the growth of endometrial stromal cells (?50% increase) from women with endometriosis compared to non-treated cells. A synergistic effect on cell proliferation was observed between exogenous Hsp70 and LPS and this was significantly suppressed by pretreatment of cells with anti-TLR4 antibody (P < 0.05). Tissue levels of Hsp70 were significantly higher in the eutopic endometria (P < 0.05) and opaque red lesions (P < 0.01) derived from women with endometriosis than from other peritoneal lesions or from women with no endometriosis. CONCLUSIONS: A prominent stress reaction was observed in blood-filled opaque red peritoneal lesions. Human Hsp70 induces pelvic inflammation and may be involved in TLR4-mediated growth of endometrial cells derived from women with endometriosis.","subitem_description_type":"Abstract"}]},"item_2_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"without figures and tables","subitem_description_type":"Other"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Human Reproduction, 23(10), pp.2210-2219; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/humrep/den195","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved."},{"subitem_rights":"This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 23(10), pp.2210-2219; 2008 is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/23/10/2210 ."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10691537","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02681161","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1460-2350","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Khan, Khaleque Newaz"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitajima, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imamura, Takehito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hiraki, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujishita, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sekine, Ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishimaru, T"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuzaki, Hideaki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"HumRep23_2210.pdf","filesize":[{"value":"96.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HumRep23_2210.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/18268/files/HumRep23_2210.pdf"},"version_id":"3d417f89-b2a9-463a-83ee-59657e92f595"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell growth","subitem_subject_scheme":"Other"},{"subitem_subject":"Endometriosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Hsp70","subitem_subject_scheme":"Other"},{"subitem_subject":"LPS","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophages","subitem_subject_scheme":"Other"},{"subitem_subject":"TLR4","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Toll-like receptor 4-mediated growth of endometriosis by human heat-shock protein 70","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Toll-like receptor 4-mediated growth of endometriosis by human heat-shock protein 70"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-08"},"publish_date":"2009-10-08","publish_status":"0","recid":"18268","relation_version_is_last":true,"title":["Toll-like receptor 4-mediated growth of endometriosis by human heat-shock protein 70"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:02:55.487065+00:00"}