@article{oai:nagasaki-u.repo.nii.ac.jp:00018293, author = {Jongwutiwes, Somchai and Putaporntip, Chaturong and Charoenkorn, Malee and Iwasaki, Takuya and Endo, Takuro}, issue = {1}, journal = {American Journal of Tropical Medicine and Hygiene}, month = {Jul}, note = {To investigate the extent of genetic heterogeneity in the genus Isospora infecting patients in Thailand, a total of 38 fecal samples containing Isospora oocysts from human immunodeficiency virus/acquired immunodeficiency syndrome patients (n = 30), corticosteroid-treated patients (n = 3) and immunocompetent individuals (n = 5) were recruited for analysis. Remarkable variation in the maximum width and length of oocysts both within and between isolates was observed. However, the average length-width ratio of oocysts was within the range for I. belli (> 1.2). Ex vivo sporogonic development of freshly passed oocysts in feces from three of these isolates was observed longitudinally, showing that 27% of these oocysts underwent complete sporulation. Interestingly, 95% of sporulated oocysts contained two sporocysts in an oocyst with four sporozoites in each sporocyst, and Caryospora-like oocysts, characterized by eight sporozoites enclosed by a single sporocyst, were also detected (5%). The small subunit ribosomal RNA (rRNA), 5.8S rRNA, internal transcribed spacer 1 (ITS-1), and ITS-2 were highly conserved, indicating that there were no cryptic species or extensive strain variation., American Journal of Tropical Medicine and Hygiene, 77(1), pp.107-112; 2007}, pages = {107--112}, title = {Morphologic and Molecular Characterization of Isospora belli Oocysts from Patients in Thailand}, volume = {77}, year = {2007} }