{"created":"2023-05-15T16:43:05.506571+00:00","id":18300,"links":{},"metadata":{"_buckets":{"deposit":"c7ddf927-0d03-4eba-82d9-97beeb741cf2"},"_deposit":{"created_by":2,"id":"18300","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"18300"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00018300","sets":["35:36"]},"author_link":["73099","73102","73097","73100","73103","73098","73101"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"93","bibliographicPageStart":"90","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"American Journal of Tropical Medicine and Hygiene"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Respiratory infections are initiated by the attachment of bacteria to pharyngeal epithelial cells. We studied the attachment of Burkholderia pseudomallei to pharyngeal epithelial cells. After one, two, three, and four washes, there were 22.6+/-8.9, 15.7+/-7.0, 6.8+/-3.1, and 4.6+/-1.1 (mean+/-SD) attached bacteria/cell, respectively. If the bacterial concentration was maintained at 1 X 10(8) colony-forming units (cfu)/ml and three washes were done, at concentrations of 2.5 x 10(4), 5 X 10(4), and 1 x 10(5) cells/ml there were 9.9+/-3.6, 3.3+/-0.8, and 2.5+/-1.1 attached bacteria/cell, respectively. If the cell concentration was kept at 2.5 x 10(4) cells/ml and three washes were done, at bacterial concentrations of 1 x 10(5), 1 X 10(6), 1 X 10(7), 1 x 10(8), and 1 x 10(9) cfu/ml, there were 0.3+/-0.3, 0.6+/-0.6, 1.0+/-0.2, 5.1+/-2.3, and 9.6+/-1.9 attached bacteria/cell, respectively. There were 4.8+/-1.9, 5.5+/-2.5, 5.6+/-1.9, and 6.4+/-2.6 attached bacteria/cell at 0, 30, 120, and 240 min of incubation, respectively. Pharyngeal cells from 10 persons (seven men and three women, mean+/-SD age = 30.7+/-8.1 years, 12 experiments with a single isolate) showed that there were 7.8+/-4.3 attached bacteria/cell. It was found that the efficiency of attachment of this bacteria was very low (7.0+/-3.3 bacteria/cell). Electron microscopy revealed that there were no fimbriae but a thin capsular polysaccharide layer on the surface of B. pseudomallei. Attachment to pharyngeal epithelial cells appeared to be mediated by this structure.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"American Journal of Tropical Medicine and Hygiene, 60(1), pp.90-93; 1999","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society of Tropical Medicine and Hygiene"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"9988329","subitem_relation_type_select":"PMID"}}]},"item_2_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.ajtmh.org/cgi/content/abstract/60/1/90"}]}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 1999 by The American Society of Tropical Medicine and Hygiene"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00521439","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00029637","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ahmed, Kamruddin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Enciso, Hernan D. R."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masaki, Hironori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tao, Misao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Omori, Akemi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tharavichikul, Prasit"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagatake, Tsuyoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"ajtmh60_90.pdf","filesize":[{"value":"245.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ajtmh60_90.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/18300/files/ajtmh60_90.pdf"},"version_id":"b827ed44-f2c7-40ee-a7be-22159e83ef98"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Attachment of Burkholderia pseudomallei to pharyngeal epithelial cells: a highly pathogenic bacteria with low attachment ability","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Attachment of Burkholderia pseudomallei to pharyngeal epithelial cells: a highly pathogenic bacteria with low attachment ability"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-21"},"publish_date":"2008-10-21","publish_status":"0","recid":"18300","relation_version_is_last":true,"title":["Attachment of Burkholderia pseudomallei to pharyngeal epithelial cells: a highly pathogenic bacteria with low attachment ability"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:01:34.069447+00:00"}