{"created":"2023-05-15T16:43:08.767378+00:00","id":18376,"links":{},"metadata":{"_buckets":{"deposit":"f0f7dd46-8272-4af3-b755-54d079bf2711"},"_deposit":{"created_by":2,"id":"18376","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"18376"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00018376","sets":["25:26"]},"author_link":["73395","73398","73397","73400","73391","73393","73392","73394","73399","73401","73396"],"item_2_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"DHMEQ, a novel NF-{kappa}B inhibitor, enhances anti-tumor activity of taxanes in anaplastic thyroid cancer cells (Author Manuscript title)"}]},"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"5365","bibliographicPageStart":"5357","bibliographicVolumeNumber":"149","bibliographic_titles":[{"bibliographic_title":"Endocrinology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nuclear factor kappaB (NF-kappaB), as an antiapoptotic factor, crucially affects the outcomes of cancer treatments, being one of the major culprits of resistance to chemotherapy. In this study, we investigated whether dehydroxymethylepoxyquinomicin (DHMEQ), a novel NF-kappaB inhibitor, can enhance antitumor activities of taxanes in anaplastic thyroid cancer (ATC) cells. Taxanes induced NF-kappaB activation in ATC cells, which could compromise the therapeutic effect of the drugs. However, DHMEQ, by inhibiting the nuclear translocation of NF-kappaB, completely suppressed the DNA binding capacities of NF-kappaB and lowered the levels of nuclear NF-kappaB protein. Compared with single treatment (either taxane or DHMEQ), the combined treatment strongly potentiated apoptosis, confirmed by cell survival assay; Western blotting for poly (ADP-ribose) polymerase, caspase 3, X-linked inhibitor of apoptosis, and survivin; and flow cytometry for annexin V. Furthermore, we also demonstrate for the first time that the combined treatment showed significantly greater inhibitory effect on tumor growth in a nude mice xenograft model. These findings suggest that taxanes are able to induce NF-kappaB activation in ATC cells, which could attenuate antitumor activities of the drugs, but inhibition of NF-kappaB by DHMEQ creates a chemosensitive environment and greatly enhances apoptosis in taxanes-treated ATC cells in vitro and in vivo. Thus, DHMEQ may emerge as an attractive therapeutic strategy to enhance the response to taxanes in ATCs.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Endocrinology, 149(11), pp.5357-5365; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Endocrine Society"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18653704","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1210/en.2008-0279","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2008 by The Endocrine Society"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00635074","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00137227","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Meng, Zhaowei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitsutake, Norisato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Starenki, Dmytro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuse, Michiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takakura, Shu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Namba, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saenko, Vladimir"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Umezawa, Kazuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohtsuru, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Shunichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"Endocrinology149_5357.pdf","filesize":[{"value":"822.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Endocrinology149_5357.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/18376/files/Endocrinology149_5357.pdf"},"version_id":"3168d4f3-a4cb-4bae-8c43-10997d6a9597"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DHMEQ","subitem_subject_scheme":"Other"},{"subitem_subject":"NF-kappaB","subitem_subject_scheme":"Other"},{"subitem_subject":"paclitaxel","subitem_subject_scheme":"Other"},{"subitem_subject":"docetaxel","subitem_subject_scheme":"Other"},{"subitem_subject":"anaplastic thyroid cancer","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dehydroxymethylepoxyquinomicin, a novel nuclear Factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dehydroxymethylepoxyquinomicin, a novel nuclear Factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells."}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-11-18"},"publish_date":"2008-11-18","publish_status":"0","recid":"18376","relation_version_is_last":true,"title":["Dehydroxymethylepoxyquinomicin, a novel nuclear Factor-kappaB inhibitor, enhances antitumor activity of taxanes in anaplastic thyroid cancer cells."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:58:25.369883+00:00"}