@article{oai:nagasaki-u.repo.nii.ac.jp:00018964,
 author = {Ishizaki(Uemura), Akiko and Sugahara, Kazuyuki and Tsuruda, Kazuto and Hasegawa, Hiroo and Yanagihara, Katsunori and Tsukasaki, Kunihiro and Yamada, Yasuaki and Kamihira, Shimeru},
 issue = {7},
 journal = {Scandinavian Journal of Clinical and Laboratory Investigation},
 month = {Nov},
 note = {All mature B-cell leukaemias and lymphomas have a clonal Ig gene recombination, and half of them have a reciprocal chromosomal translocation involving the 14q32 locus. The 14q32 translocation partners are variable, such as BCL-2, BCL-1 and BCL-6, thus accounting for the difficulty in molecular detection by the current genomic polymerase chain reaction (PCR) method. To identify B-cell clones efficiently with an Ig gene rearrangement and reciprocal inter-chromosomal translocation, we verified the usefulness, in a practical laboratory setting, of our modified long-distance inverse (LDI) PCR method for detecting IgH gene rearrangements involving inter- and intra-chromosomal segments. The total run time of this LDI PCR method was 5.5 h. Using 24 samples of mature B-cell leukaemias and lymphomas, the modified LDI PCR gave clonally rearranged amplicons in 83 % (20/24) of cases. Direct sequencing results of the amplicons revealed inter-chromosomal translocations in 5 cases (25 %) and intra-chromosomal rearrangements in the remaining 15 cases (75 %). The partners of the inter-chromosomal translocation consisted of the 11q13.3 segment containing a partial BCL1 sequence in 3 cases; 18q21.3 segment containing a partial BCL2 sequence in one case; and a segment of 7q11.2 in one case. We present an LDI PCR-based methodology for the efficient identification of 14q32 translocations, with modifications to reduce the total run time to within one day., Scandinavian Journal of Clinical and Laboratory Investigation, 68(7), pp.519-525; 2008},
 pages = {519--525},
 title = {Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting.},
 volume = {68},
 year = {2008}
}