{"created":"2023-05-15T16:43:34.368127+00:00","id":18964,"links":{},"metadata":{"_buckets":{"deposit":"7b1d930b-d7ec-488d-b5ad-83c726433655"},"_deposit":{"created_by":2,"id":"18964","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"18964"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00018964","sets":["29:30"]},"author_link":["77754","77757","77758","77753","77756","77752","77751","77755"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"525","bibliographicPageStart":"519","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Scandinavian Journal of Clinical and Laboratory Investigation"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"All mature B-cell leukaemias and lymphomas have a clonal Ig gene recombination, and half of them have a reciprocal chromosomal translocation involving the 14q32 locus. The 14q32 translocation partners are variable, such as BCL-2, BCL-1 and BCL-6, thus accounting for the difficulty in molecular detection by the current genomic polymerase chain reaction (PCR) method. To identify B-cell clones efficiently with an Ig gene rearrangement and reciprocal inter-chromosomal translocation, we verified the usefulness, in a practical laboratory setting, of our modified long-distance inverse (LDI) PCR method for detecting IgH gene rearrangements involving inter- and intra-chromosomal segments. The total run time of this LDI PCR method was 5.5 h. Using 24 samples of mature B-cell leukaemias and lymphomas, the modified LDI PCR gave clonally rearranged amplicons in 83 % (20/24) of cases. Direct sequencing results of the amplicons revealed inter-chromosomal translocations in 5 cases (25 %) and intra-chromosomal rearrangements in the remaining 15 cases (75 %). The partners of the inter-chromosomal translocation consisted of the 11q13.3 segment containing a partial BCL1 sequence in 3 cases; 18q21.3 segment containing a partial BCL2 sequence in one case; and a segment of 7q11.2 in one case. We present an LDI PCR-based methodology for the efficient identification of 14q32 translocations, with modifications to reduce the total run time to within one day.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Scandinavian Journal of Clinical and Laboratory Investigation, 68(7), pp.519-525; 2008","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Informa Healthcare"}]},"item_2_relation_11":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18609098","subitem_relation_type_select":"PMID"}}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/00365510701858240","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2008 Informa plc"},{"subitem_rights":"This is an electronic version of an article published in  Scandinavian Journal of Clinical and Laboratory Investigation, 68(7), 519-525: 2008 November. Scandinavian Journal of Clinical and Laboratory Investigation is available online at: http://www.informaworld.com/openurl?genre=article&issn=00365513&volume=68&issue=7&spage=519."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00365513","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"1502-7686","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishizaki(Uemura), Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugahara, Kazuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuruda, Kazuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Hiroo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagihara, Katsunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsukasaki, Kunihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Yasuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamihira, Shimeru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"SJCLI68_519.pdf","filesize":[{"value":"332.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SJCLI68_519.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/18964/files/SJCLI68_519.pdf"},"version_id":"e58d4b64-a826-408a-995f-c12da35a9a62"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"B-cell clonality","subitem_subject_scheme":"Other"},{"subitem_subject":"IgH gene","subitem_subject_scheme":"Other"},{"subitem_subject":"lymphoma","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting."}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-03"},"publish_date":"2009-12-03","publish_status":"0","recid":"18964","relation_version_is_last":true,"title":["Usefulness of long-distance inverse polymerase chain reaction for molecular detection of 14q32 translocation in a clinical setting."],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:37:05.602294+00:00"}