@article{oai:nagasaki-u.repo.nii.ac.jp:00019014,
 author = {Ahmed, Sameh and Kishikawa, Naoya and Ohyama, Kaname and Wada, Mitsuhiro and Nakashima, Kenichiro and Kuroda, Naotaka},
 issue = {1},
 journal = {Talanta},
 month = {Apr},
 note = {A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in the presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-μL was sufficient. Besides, it can detect accurately the low plasma concentrations. The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7?102.4% and the inter- and intra-assay RSD values in rat plasma were 2.5?8.9%. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single-dose of DXR., Talanta, 78(1), pp.94-100; 2009},
 pages = {94--100},
 title = {Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection},
 volume = {78},
 year = {2009}
}