{"created":"2023-05-15T16:43:36.537921+00:00","id":19014,"links":{},"metadata":{"_buckets":{"deposit":"0cff7670-d260-4a6a-ae3e-2b22ba53d61c"},"_deposit":{"created_by":2,"id":"19014","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"19014"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00019014","sets":["10:11"]},"author_link":["78054","78056","78053","78055","78057","78052"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-04-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"100","bibliographicPageStart":"94","bibliographicVolumeNumber":"78","bibliographic_titles":[{"bibliographic_title":"Talanta"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in the presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-μL was sufficient. Besides, it can detect accurately the low plasma concentrations. The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7?102.4% and the inter- and intra-assay RSD values in rat plasma were 2.5?8.9%. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single-dose of DXR.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Talanta, 78(1), pp.94-100; 2009","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.talanta.2008.10.043","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright c 2008 Elsevier B.V. All rights reserved."}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11539538","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00399140","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ahmed, Sameh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Mitsuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"Talanta78_94.pdf","filesize":[{"value":"648.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Talanta78_94.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/19014/files/Talanta78_94.pdf"},"version_id":"9178ccf0-4488-4f31-ad14-e6698b01a272"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Doxorubicin","subitem_subject_scheme":"Other"},{"subitem_subject":"Doxorubicinol","subitem_subject_scheme":"Other"},{"subitem_subject":"Pharmacokinetics","subitem_subject_scheme":"Other"},{"subitem_subject":"Peroxyoxalate chemiluminescence detection","subitem_subject_scheme":"Other"},{"subitem_subject":"Photosensitization","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-01-08"},"publish_date":"2009-01-08","publish_status":"0","recid":"19014","relation_version_is_last":true,"title":["Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:35:02.921754+00:00"}