{"created":"2023-05-15T16:30:41.578614+00:00","id":1915,"links":{},"metadata":{"_buckets":{"deposit":"a8731665-6992-4e22-bc5f-e4710c702da0"},"_deposit":{"created_by":2,"id":"1915","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"1915"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00001915","sets":["35:36"]},"author_link":["8838","8840","8839","8842","8837","8841"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"193","bibliographicPageStart":"186","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Parasitology International"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Plasmodium falciparum, an obligate intracellular protozoan parasite which causes the severe form of human malaria, \nexports numerous proteins to the infected red blood cell that are important for its survival and of severe pathological effect to its host. These proteins and their export mechanisms are candidates for drug \nand vaccine development, and among them is the Plasmodium SURFIN family of proteins. Previously we showed that the N-terminal region along with the sequence surrounding the transmembrane domain of SURFIN 4.1 is essential for its export to Maurer's clefts in the red blood cell cytoplasm. We proposed that this region is recognized by a machinery responsible for protein translocation across the parasitophorous vacuole membrane surrounding the parasite. \nTo understand the export mechanism further, we utilized a fluorescent protein-tagged mini-SURFIN 4.1 consisting of the minimum essential components for export. Alanine scanning of all charged amino acids \nwithin the N-terminal region revealed that replacement of 3 glutamic acid and 2 lysine residues significantly impairs the export efficiency of this protein across the parasitophorous vacuole membrane. In addition,N-terminally Myc-tagged mini-SURFIN 4.1 and mini-SURFIN 4.2 with similar architectures were detected with anti-Myc antibody at Maurer's clefts, indicating that elements required for export to Maurer's clefts are conserved between SURFIN 4.1 and SURFIN 4.2 , and that N-terminal sequences of these SURFIN members are not cleaved during export. Our results implicate a conserved nature of SURFIN export \nto the red blood cell, particularly an important role of multiple glutamic acid and lysine residues in the SURFIN N-terminal region.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Parasitology International, 71, pp.186-193; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.parint.2019.04.019","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13835769","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chitama, Ben-Yeddy Abel"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyazaki, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhu, Xiaotong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagaya, Wataru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yahata, Kazuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Osamu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"ParInt71_186.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ParInt71_186.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/1915/files/ParInt71_186.pdf"},"version_id":"cb86e83c-5e8c-4517-894a-6af1011139ee"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Erythrocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"Malaria","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmodium falciparum","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein export","subitem_subject_scheme":"Other"},{"subitem_subject":"SURFIN","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Multiple charged amino acids of Plasmodium falciparum SURFIN4.1 N-terminal region are important for efficient export to the red blood cell","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Multiple charged amino acids of Plasmodium falciparum SURFIN4.1 N-terminal region are important for efficient export to the red blood cell"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-24"},"publish_date":"2020-04-24","publish_status":"0","recid":"1915","relation_version_is_last":true,"title":["Multiple charged amino acids of Plasmodium falciparum SURFIN4.1 N-terminal region are important for efficient export to the red blood cell"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:55:11.537042+00:00"}