@article{oai:nagasaki-u.repo.nii.ac.jp:00019403,
 author = {Kagawa, Yasuhiro and Yatagai, Fumio and Suzuki, Masao and Kase, Youko and Kobayashi, Akiko and Hirano, Masahiko and Kato, Takesi and Watanabe, Masami and Hanaoka, Fumio},
 issue = {3},
 journal = {Journal of radiation research},
 month = {Sep},
 note = {Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing., Journal of radiation research. 1995, 36(3), p.185-195},
 pages = {185--195},
 title = {Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation},
 volume = {36},
 year = {1995}
}