{"created":"2023-05-15T16:43:53.391693+00:00","id":19403,"links":{},"metadata":{"_buckets":{"deposit":"876ca2d2-0089-4267-8538-adca5e4e4a6f"},"_deposit":{"created_by":2,"id":"19403","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"19403"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00019403","sets":["73:74"]},"author_link":["80833","80835","80834","80832","80829","80831","80828","80830","80836","80837"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"195","bibliographicPageStart":"185","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Journal of radiation research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of radiation research. 1995, 36(3), p.185-195","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"80837","nameIdentifierScheme":"WEKO"}],"names":[{"name":"谷田貝, 文夫"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本放射線影響学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本放射線影響学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00705792","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04493060","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kagawa, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yatagai, Fumio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kase, Youko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Masahiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Takesi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Masami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hanaoka, Fumio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"RR36_3_185_195.pdf","filesize":[{"value":"494.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"RR36_3_185_195.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/19403/files/RR36_3_185_195.pdf"},"version_id":"4c1a59b2-ae5f-4f4d-83b6-935e8831b486"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"HPRT","subitem_subject_scheme":"Other"},{"subitem_subject":"Heavy-Ion Irradiation","subitem_subject_scheme":"Other"},{"subitem_subject":"Aberrant mRNA Splicing","subitem_subject_scheme":"Other"},{"subitem_subject":"High LET","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-07-24"},"publish_date":"2008-07-24","publish_status":"0","recid":"19403","relation_version_is_last":true,"title":["Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:19:09.612934+00:00"}