@article{oai:nagasaki-u.repo.nii.ac.jp:00019414,
 author = {Satoh, Akiko and Yokozawa, Takako and Tanaka, Takashi and Okamoto, Takuya and Sei, Yasuo},
 issue = {2},
 journal = {和漢医薬学雑誌},
 month = {Apr},
 note = {Replicative senescence (RS) of human diploid fibro blasts (HDFs) has become a classical model of aging and HDFs, such as WI-38 cells, display increased cellular oxidant production associated with RS. Several phenomena associated with RS are also observed in stress-induced replicative senescence (SIPS). In particular, SIPS of WI-38 cells caused by hydrogen peroxide (H_2O_2) is a useful and reasonable cellular aging model for evaluating the effects of potential anti-aging agents against oxidative stress. We used this well-established model to evaluate the anti-aging effect of Kangen-karyu, focusing on its antioxidant activity. Treatment of WI-38 cells undergoing SIPS caused by H_20_2 with Kangen-karyu extract significantly reduced reactive oxygen species (ROS) generation and lipid peroxidation levels. In addition, the intracellular GSH levels, reflecting cellular ROS generation, were reduced by treatment with Kangen-karyu extract. These results suggest that Kangen-karyu attenuated the age-associated increase of cellular oxidative damage. Moreover, Kangen-karyu extract normalized the G_0/G_1 phase arrest and reversed the diminished cell viability resulting from exposure to H_2O_2. Furthermore, the extract prolonged the lifespan of WI-38 cells undergoing SIPS. This study suggests that Kangen-karyu may delay the aging process in cells undergoing SIPS by attenuating oxidative damage., 和漢医薬学雑誌. 2004, 21(2), p.87-93},
 pages = {87--93},
 title = {The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts},
 volume = {21},
 year = {2004}
}