{"created":"2023-05-15T16:43:53.868086+00:00","id":19414,"links":{},"metadata":{"_buckets":{"deposit":"24279439-3d5d-4e3e-bea5-d16ffa5ec162"},"_deposit":{"created_by":2,"id":"19414","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"19414"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00019414","sets":["73:74"]},"author_link":["80903","80907","80906","80904","80905","80902"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-04-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"93","bibliographicPageStart":"87","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"和漢医薬学雑誌"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Replicative senescence (RS) of human diploid fibro blasts (HDFs) has become a classical model of aging and HDFs, such as WI-38 cells, display increased cellular oxidant production associated with RS. Several phenomena associated with RS are also observed in stress-induced replicative senescence (SIPS). In particular, SIPS of WI-38 cells caused by hydrogen peroxide (H_2O_2) is a useful and reasonable cellular aging model for evaluating the effects of potential anti-aging agents against oxidative stress. We used this well-established model to evaluate the anti-aging effect of Kangen-karyu, focusing on its antioxidant activity. Treatment of WI-38 cells undergoing SIPS caused by H_20_2 with Kangen-karyu extract significantly reduced reactive oxygen species (ROS) generation and lipid peroxidation levels. In addition, the intracellular GSH levels, reflecting cellular ROS generation, were reduced by treatment with Kangen-karyu extract. These results suggest that Kangen-karyu attenuated the age-associated increase of cellular oxidative damage. Moreover, Kangen-karyu extract normalized the G_0/G_1 phase arrest and reversed the diminished cell viability resulting from exposure to H_2O_2. Furthermore, the extract prolonged the lifespan of WI-38 cells undergoing SIPS. This study suggests that Kangen-karyu may delay the aging process in cells undergoing SIPS by attenuating oxidative damage.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"和漢医薬学雑誌. 2004, 21(2), p.87-93","subitem_description_type":"Other"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"80907","nameIdentifierScheme":"WEKO"}],"names":[{"name":"横澤, 隆子"}]}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"和漢医薬学会"}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"和漢医薬学会"},{"subitem_rights":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12035198","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18801447","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Satoh, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokozawa, Takako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sei, Yasuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-23"}],"displaytype":"detail","filename":"wakan21_2_87_93.pdf","filesize":[{"value":"858.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"wakan21_2_87_93.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/19414/files/wakan21_2_87_93.pdf"},"version_id":"7e34e6ea-e286-45b7-8057-28f75b06f01f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Kangen-karyu","subitem_subject_scheme":"Other"},{"subitem_subject":"aging","subitem_subject_scheme":"Other"},{"subitem_subject":"WI-38","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrogen peroxide","subitem_subject_scheme":"Other"},{"subitem_subject":"free radical","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-07-24"},"publish_date":"2008-07-24","publish_status":"0","recid":"19414","relation_version_is_last":true,"title":["The antioxidative activity of Kangen-karyu extract delays senescence of human lung fibroblasts"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:18:39.464355+00:00"}