@article{oai:nagasaki-u.repo.nii.ac.jp:00019423,
 author = {森井, 秀昭 and 笠間, 憲太郎 and 高水, 智宏 and 城島, 一将},
 journal = {長崎大学水産学部研究報告, Bulletin of the Faculty of Fisheries, Nagasaki University},
 month = {Mar},
 note = {In order lo study whether the psychrotrophic Pseudomonas isolates showing different growth curves are composed of different ecotype, cellular proteins of the non-halophilic psychrotrophs during growth were determined by two-dimensional gel electrophoresis using isoelectric focusing in one dimension and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) in the second dimension. Growth curves of the psychrotrophs showed an increased slope throughout growth (in Type 1 strains) or a decreased slope after the stationary phase of growth (in Type 2 strains) under optimum temperatures. Protein bands on both isoelectric focusing gels and SDS gels and protein spots on the two-dimensional gels were very clear for Type 1 strains and unclear (become foggy) for Type 2 strains, suggesting that the protein molecules have compact structure in Type 1 strains and deformable structure in Type 2 strains. The number and amounts of the protein spots increased in Type 1 strains during growth and decreased in Type 2 strains during the death phase of growth at the optimum temperatures. That is, the difference of growth curves between Type 1 and 2 strains was considered to be based on the difference of physical and chemical structures for tertiary folding of protein molecules. In other words, it was suggested that the two Type strains of the psychrotrophic Pseudomonas isolates are composed of different ecotype. In Type 2 strains, moreover, growth curves showed an increased slope throughout growth under 1O"C, the number and amounts of the protein spots increased during growth atlOC, and the protein bands and spots were more clear in the strains grown under 1OC than those grown under the optimum temperature, suggesting that the molecules have more compact structure in the strains grown under 1O"C. These facts suggest that Type 2 strain have evolved to be adapted to more cold. In addition, the protein spots obtained on the twodimensional gels were very different between eBB and silver stainings and were more observed in the acidic side in the former and in the neutral side in the latter., 長崎大学水産学部研究報告 v.88 p.81-94; 2007},
 pages = {81--94},
 title = {生育パターンの異なる低温性 Pseudomonas属細菌の生育に伴う菌体タンパク質の変動},
 volume = {88},
 year = {2007}
}