@article{oai:nagasaki-u.repo.nii.ac.jp:00019613,
 author = {田浦, 保穂 and 角田, 司 and 塩竃, 利昭 and Mullen, Yoko and Papoian, Tom},
 issue = {3},
 journal = {The journal of veterinary medical science},
 month = {Jun},
 note = {ミニブタの同種移植における免疫反応の指標を得る目的で, ドナー末梢血リンパ球(PBL)前処置した後, ドナーPBLに対する in vitroでのIL-2様活性の測定を行い, 以下の結果が得られた: (1)MHC不適合無処置群の48時間MLC上清中にIL-2様活性がみられ, 48時間培養の方が高値であった; (2)MHC不適合PBLを1回移植した群は, 無処置群よりも in vitroでのIL-2様活性は高値であった; (3)MHC不適合PBLを週1回計4回移植した群は, in vitroでのIL-2様活性の検出がやや早期に認められた., In order to determine the means of monitoring the immunological status of allograft recipients in miniature swine, an assay was developed to measure interleukin (IL)-2 production in vitro by pretreatment of donor peripheral blood lymphocytes (PBL). Miniature swine were given 0 to 4 weekly intravenous transfusions of 5-10×10^7 donor PBL incompatible at major histocompatibility complex (MHC) and assayed in vitro for donor specific immune IL-2-like activities. The results are summarized as follows: (1) IL-2-like activity in 24 hr and 48 hr supernatants from mixed lymphocyte cultures (MLC) with MHC-incompatible PBL was detected without pretreatment. The 48 hr MLC supernatant exhibited a high IL-2-like activity compared with the 24 hr; (2) IL-2-like activity after only one transfusion with MHC-incompatible PBL was higher than that without pretreatment; (3) IL-2-like activity in 4 weekly transfusions was detectable slightly earlier than that without pretreatment or three transfusions with MHC-incompatibile PBL., The journal of veterinary medical science. 1991, 53(3), p.365-369},
 pages = {365--369},
 title = {ミニブタの同種末梢血リンパ球前処置による in vitro IL-2様活性の早期検出},
 volume = {53},
 year = {1991}
}