@article{oai:nagasaki-u.repo.nii.ac.jp:00001993,
 author = {Murakami, Naoko and Kitajima, Michio and Ohyama, Kaname and Aibara, Nozomi and Taniguchi, Ken and Wei, Mian and Kitajima, Yuriko and Miura, Kiyonori and Masuzaki, Hideaki},
 journal = {Clinica Chimica Acta},
 month = {May},
 note = {Background: Autoimmune reactions and subsequent inflammation may underlie spermatogenic dysfunction and endometriosis-related infertility.The aim of this study is to identify disease-specific antigens in immune complexes (ICs) in seminal plasma (SP) and in follicular fluid (FF).
Methods: Immune complexome analysis, in which nano-liquid chromatography-tandem mass spectrometry is employed to comprehensively identify antigens incorporated into ICs in biological fluids, was performed for specimens collected from infertile couples undergoing assisted reproduction.Forty-two male patients consisting of subjects with oligozoospermia (n=6), asthenozoospermia (n=8), and normal semen analysis (n=28).
 Fifty-eight female patients consisting of subjects with ovarian endometriosis (n=10) and control women without disease (n=48).
Results: Four disease-specific antigens were identified in subjects with oligozoospermia, while five disease-specific antigens were detected in subjects with asthenozoospermia, some of which are involved in sprematogenesis. Eight antigens were detected only in subjects with endometriosis.
Conclusion: Functional characteristics of disease-specific antigens were found to correspond to the pathogenesis of male and female infertility. The formation of ICs may contribute to spermatogenic dysfunction and endometriosis-related infertility via loss of function of the related proteins.
 Immune complexome analysis is expected to be a valuable tool for the investigation of novel diagnostic methods and treatment strategies for infertility., Clinica Chimica Acta, 495, pp.545-551; 2019},
 pages = {545--551},
 title = {Comprehensive immune complexome analysis detects disease-specific immune complex antigens in seminal plasma and follicular fluids derived from infertile men and women},
 volume = {495},
 year = {2019}
}