{"created":"2023-07-11T02:06:07.623205+00:00","id":2000020,"links":{},"metadata":{"_buckets":{"deposit":"d0b6e4cd-1298-47c7-a892-316983ff0ae0"},"_deposit":{"created_by":6,"id":"2000020","owner":"6","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2000020"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:02000020","sets":["8:9"]},"author_link":[],"control_number":"2000020","item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"194","bibliographicPageStart":"187","bibliographicVolumeNumber":"665","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The autonomic nervous system plays an important role in the regulation of peripheral inflammation. Sympathetic nervous activation stimulates inflammatory gene expression and cytokines, whereas parasympathetic nervous activation suppresses the production of inflammatory cytokines by immune cells. However, most studies on the relationship between the autonomic nervous system and immune processes have analyzed a single branch of the autonomic nerves in isolation. Therefore, this study aimed to examine the effects of sympathetic and parasympathetic stimulation on macrophages, which are controlled by autonomic regulation. Macrophages were stimulated with lipopolysaccharide (LPS) to induce TNF-a. Then, the effects of b2 adrenergic receptor and a7 nicotinic acetylcholine receptor activation on TNF-a production were assessed using concentration-dependent assays. RNA-seq data were also used to identify genes whose expression was enhanced by parasympathetic and sympathetic stimulation. The simultaneous activation of b2 adrenergic receptors and a7 nicotinic acetylcholine receptors suppressed LPS-induced TNF-a production in a concentration-dependent manner. Moreover, simultaneous activation of these receptors had synergistic anti-inflammatory effects and induced Tspan13 expression, thereby contributing to anti-inflammatory mechanisms in macrophages. Our study revealed the synergistic anti-inflammatory effects of the parasympathetic and sympathetic stimulation of macrophages. Our results suggest that targeting both sympathetic and parasympathetic signaling is a promising therapeutic approach for inflammatory diseases.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and Biophysical Research Communications, 665, pp.187-194; 2023","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V.","subitem_publisher_language":"en"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2023.04.118","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2023 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Umene, Ryusuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakamura, Yasuna","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Wu, Chia-Hsien","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Muta, Kumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nishino, Tomoya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Tsuyoshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-05-08"}],"displaytype":"detail","filename":"BBRC665_187.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://nagasaki-u.repo.nii.ac.jp/record/2000020/files/BBRC665_187.pdf"},"version_id":"b1f51995-1769-44f3-a955-aca0fa544383"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Neuro-immune system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Synergistic anti-inflammatory effect","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cholinergic anti-inflammatory pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"a7 nicotinic acetylcholine receptors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"b2 adrenergic receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tspan13","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Induction of tetraspanin 13 contributes to the synergistic anti-inflammatory effects of parasympathetic and sympathetic stimulation in macrophages","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Induction of tetraspanin 13 contributes to the synergistic anti-inflammatory effects of parasympathetic and sympathetic stimulation in macrophages","subitem_title_language":"en"}]},"item_type_id":"2","owner":"6","path":["9"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-07-11"},"publish_date":"2023-07-11","publish_status":"0","recid":"2000020","relation_version_is_last":true,"title":["Induction of tetraspanin 13 contributes to the synergistic anti-inflammatory effects of parasympathetic and sympathetic stimulation in macrophages"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-07-11T07:46:40.429488+00:00"}