{"created":"2023-07-13T04:17:08.301554+00:00","id":2000036,"links":{},"metadata":{"_buckets":{"deposit":"0080cefb-90d4-4636-b1a3-e4f139644c69"},"_deposit":{"created_by":6,"id":"2000036","owner":"6","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2000036"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:02000036","sets":["10:11"]},"author_link":[],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05-19","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"110","bibliographicPageStart":"104","bibliographicVolumeNumber":"667","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Biomechanical forces are known to regulate the biological behaviors of cells. Although negative pressure has been used for wound healing, it is still unknown about its role in regulating cell plasticity. We investigated whether negative pressure could induce the dedifferentiation of hepatocytes. Using a commercial device, we found that the exposure of primary human hepatocytes to −50 mmHg quickly induced the formation of stress fibers and obviously changed cell morphology in 72 h. Moreover, the exposure of hepatocytes to −50 mmHg significantly upregulated RhoA, ROCK1, and ROCK2 in 1–6 h, and dramatically enhanced the expression of marker molecules on “stemness”, such as OCT4, SOX2, KLF4, MYC, NANOG, and CD133 in 6–72 h. However, all these changes in hepatocytes induced by −50 mmHg stimulation were almost abrogated by ROCK inhibitor Y27623. Our data suggest that an appropriate force of negative pressure stimulation can effectively induce the dedifferentiation of hepatocytes via RhoA/ROCK pathway activation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biochemical and Biophysical Research Communications, 667, pp.104-110; 2023","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V.","subitem_publisher_language":"en"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbrc.2023.05.042","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights_language":"en","subitem_rights_resource":"© 2023 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Khalifa, Mahmoud Osman","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Moriwaki, Takahito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Zhang, Shouhua","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Zhou, Wei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ito, Kosei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Li, Tao-Sheng","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2024-05-19"}],"filename":"BBRC667_104.pdf","filesize":[{"value":"941 KB"}],"format":"application/pdf","url":{"url":"https://nagasaki-u.repo.nii.ac.jp/record/2000036/files/BBRC667_104.pdf"},"version_id":"04449f85-f9c2-423f-ba5a-f232d2f2fe35"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hepatocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Dedifferentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stemness","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RhoA/ROCK","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Negative pressure","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway","subitem_title_language":"en"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-07-13"},"publish_date":"2023-07-13","publish_status":"0","recid":"2000036","relation_version_is_last":true,"title":["Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-07-13T04:26:42.685962+00:00"}