{"created":"2024-02-08T02:43:58.881764+00:00","id":2000567,"links":{},"metadata":{"_buckets":{"deposit":"46bd12ef-1df0-4d87-b8d7-756ea2bbbdaa"},"_deposit":{"created_by":6,"id":"2000567","owner":"6","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2000567"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:02000567","sets":["6:7"]},"author_link":[],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-12-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"art. no. e0190523","bibliographicVolumeNumber":"90","bibliographic_titles":[{"bibliographic_title":"Applied and Environmental Microbiology","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A moderately halophilic eubacterium, Halomonas elongata, has been used as cell factory to produce fine chemical 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine), which functions as a major osmolyte protecting the cells from high-salinity stress. To explore the possibility of using H. elongata to biosynthesize other valuable osmolytes, an ectoine-deficient salt-sensitive H. elongata deletion mutant strain KA1 (ΔectABC), which only grows well in minimal medium containing up to 3% NaCl, was subjected to an adaptive mutagenesis screening in search of mutants with restored salt tolerance. Consequently, we obtained a mutant, which tolerates 6% NaCl in minimal medium by overproducing L-glutamic acid (Glu). However, this Glu-overproducing (GOP) strain has a lower tolerance level than the wild-type H. elongata, possibly because the acidity of Glu interferes with the pH homeostasis of the cell and hinders its own cellular accumulation. Enzymatic decarboxylation of Glu to γ-aminobutyric acid (GABA) by a Glu decarboxylase (GAD) could restore cellular pH homeostasis; therefore, we introduced an engineered salt-inducible HopgadBmut gene, which encodes a wide pH-range GAD mutant, into the genome of the H. elongata GOP strain. We found that the resulting H. elongata GOP-Gad strain exhibits higher salt tolerance than the GOP strain by accumulating high concentration of GABA as an osmolyte in the cell (176.94 µmol/g cell dry weight in minimal medium containing 7% NaCl). With H. elongata OUT30018 genetic background, H. elongata GOP-Gad strain can utilize biomass-derived carbon and nitrogen compounds as its sole carbon and nitrogen sources, making it a good candidate for the development of GABA-producing cell factories.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Applied and environmental microbiology, 90(1), art. no. e0190523; 2023","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology","subitem_publisher_language":"en"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/aem.01905-23","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© American Society for Microbiology, Applied and environmental microbiology, 90(1), art. no. e0190523; 2023.","subitem_rights_language":"en"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0099-2240","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zou, Ziyan","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kaothien-Nakayama, Pulla","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ogawa-Iwamura, Junpei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakayama, Hideki","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-02-08"}],"filename":"AEM90_e0190523.pdf","filesize":[{"value":"869 KB"}],"format":"application/pdf","url":{"url":"https://nagasaki-u.repo.nii.ac.jp/record/2000567/files/AEM90_e0190523.pdf"},"version_id":"2ec2510d-86ed-4273-aaef-22f68d031372"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Halomonas elongata","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"compatible osmolyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"L-glutamic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolic engineering","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"γ-aminobutyric acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Metabolic engineering of high-salinity-induced biosynthesis of γ-aminobutyric acid improves salt-stress tolerance in a glutamic acid-overproducing mutant of an ectoine-deficient            Halomonas elongata","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Metabolic engineering of high-salinity-induced biosynthesis of γ-aminobutyric acid improves salt-stress tolerance in a glutamic acid-overproducing mutant of an ectoine-deficient            Halomonas elongata","subitem_title_language":"en"}]},"item_type_id":"2","owner":"6","path":["7"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-02-08"},"publish_date":"2024-02-08","publish_status":"0","recid":"2000567","relation_version_is_last":true,"title":["Metabolic engineering of high-salinity-induced biosynthesis of γ-aminobutyric acid improves salt-stress tolerance in a glutamic acid-overproducing mutant of an ectoine-deficient            Halomonas elongata"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2024-02-08T02:48:10.575951+00:00"}