{"created":"2024-04-11T01:47:52.757201+00:00","id":2000721,"links":{},"metadata":{"_buckets":{"deposit":"5a9e184c-b96c-4f27-9432-5d79f6914859"},"_deposit":{"created_by":6,"id":"2000721","owner":"6","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2000721"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:02000721","sets":["10:11"]},"author_link":[],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"art. no. 12","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Laboratory Animal Research","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Although radiotherapy is commonly used to treat head and neck cancer, it may lead to radiation-associated dysphagia (RAD). There are various causes of RAD, however, the mechanism has not yet been fully identified. Currently, the only effective treatment for RAD is rehabilitation. Additionally, there are few available animal models of RAD, necessitating the development of new models to establish and evaluate RAD treatments. We hypothesize that radiation-induced neck muscle fibrosis could be one of the causes of RAD due to impairment of laryngeal elevation. Therefore, in this study, we focused on the changes in inflammation and fibrosis of the strap muscles (Sternohyoid, Sternothyroid, and Thyrohyoid muscles) after a single-dose irradiation. This research aims to provide a reference animal model for future studies on RAD. Results: Compared to control mice, those treated with 72-Gy, but not 24-Gy, irradiation had significantly increased tumor necrosis factor-α (TNF-α) (p < 0.01) and α-smooth muscle actin (αSMA) (p < 0.05) expression at 10 days and significantly increased expression levels of motif chemokine ligand-2 (CCL2), α-SMA, tumor growth factor-β1 (TGF-β1), type1 collagen, and interleukin-1β (IL-1β) (p < 0.05) in the muscles at 1 month by real-time PCR analysis. The results of immunohistochemistry showed that the deposition of type 1 collagen gradually increased in extracellular space after radiation exposure, and the positive area was significantly increased at 3 months compared to non-irradiated control. Conclusions: A single dose of 72-Gy irradiation induced significant inflammation and fibrosis in the strap muscles of mice at 1 month, with immunohistochemical changes becoming evident at 3 months. This cervical irradiationinduced fibrosis model holds potential for establishing an animal model for RAD in future studies. Level of evidence: N/A.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Laboratory Animal Research, 40(1), art. no. 12; 2024","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd","subitem_publisher_language":"en"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s42826-024-00199-2","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.","subitem_rights_language":"en"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2233-7660","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Soejima, Shuntaro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Wu, Chia-Hsien","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Matsuse, Haruna","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Terakado, Mariko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Okano, Shinji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Tsuyoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kumai, Yoshihiko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-04-11"}],"filename":"LAR40_12.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","url":{"url":"https://nagasaki-u.repo.nii.ac.jp/record/2000721/files/LAR40_12.pdf"},"version_id":"90d69fe3-b581-4d49-a659-8b5e60d41928"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Radiation-induced fibrosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Radiation-associated dysphagia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Animal model","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mouse","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Strap muscle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Swallowing-related muscle inflammation and fibrosis induced by a single dose of radiation exposure in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Swallowing-related muscle inflammation and fibrosis induced by a single dose of radiation exposure in mice","subitem_title_language":"en"}]},"item_type_id":"2","owner":"6","path":["11"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-11"},"publish_date":"2024-04-11","publish_status":"0","recid":"2000721","relation_version_is_last":true,"title":["Swallowing-related muscle inflammation and fibrosis induced by a single dose of radiation exposure in mice"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2024-04-11T01:52:51.566894+00:00"}