{"created":"2024-04-16T01:04:01.916102+00:00","id":2000726,"links":{},"metadata":{"_buckets":{"deposit":"a76162b2-46b9-47ff-80ce-a600c4b5d735"},"_deposit":{"created_by":6,"id":"2000726","owner":"6","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"2000726"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:02000726","sets":["35:36"]},"author_link":[],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-04-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"art. no. 30","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"Tropical Medicine and Health","bibliographic_titleLang":"en"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: There is a lack of data on the characteristics of overlap between acquired antimicrobial resistance and virulence factors in Klebsiella pneumoniae in high-risk settings, especially with the inclusion of surveillance isolates along with the clinical. We investigated K. pneumoniae isolates, from a neonatal intensive care unit (NICU) in Nepal, for the presence of both accessory virulence factors and acquired antimicrobial resistance. Methods: Thirty-eight clinical and nineteen surveillance K. pneumoniae isolates obtained between January 2017 and August 2022 in the NICU of Siddhi Memorial Hospital, Bhaktapur, Nepal were investigated with antimicrobial susceptibility testing, PCR-based detection of β-lactamases and virulence factors, and genetic similarity by ERIC–PCR. Results: K. pneumoniae was found positive in 37/85 (43.5%) blood culture-positive neonatal bloodstream infections, 34/954 (3.6%) patient surveillance cultures, and 15/451 (3.3%) environmental surveillance samples. Among 57 isolates analyzed in this study, we detected multidrug resistance in 37/57 (64.9%), which was combined with at least one accessory virulence factor in 21/37 (56.8%). This overlap was mostly among β-lactamase producing isolates with accessory mechanisms of iron acquisition. These isolates displayed heterogenous ERIC–PCR patterns suggesting genetic diversity. Conclusions: The clinical significance of this overlap between acquired antimicrobial resistance and accessory virulence genes in K. pneumoniae needs further investigation. Better resource allocation is necessary to strengthen infection prevention and control interventions in resource-limited settings.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Tropical Medicine and Health, 52(1), art. no. 30; 2024","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd","subitem_publisher_language":"en"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/s41182-024-00595-3","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1349-4147","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shrestha, Raj Kumar","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shrestha, Dhruba","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kunwar, Ajaya Jang","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Thapa, Sandeep","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shrestha, Nipun","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Dhoubhadel, Bhim Gopal","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Parry, Christopher M.","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-04-16"}],"filename":"TMH52_30.pdf","filesize":[{"value":"1006 KB"}],"format":"application/pdf","url":{"url":"https://nagasaki-u.repo.nii.ac.jp/record/2000726/files/TMH52_30.pdf"},"version_id":"00ef7224-3ed3-4adb-a90b-5dc5ac442579"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Extended-spectrum β-lactamase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Klebsiella pneumoniae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neonatal intensive care unit (NICU)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nepal","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Virulence","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting","subitem_title_language":"en"}]},"item_type_id":"2","owner":"6","path":["36"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-16"},"publish_date":"2024-04-16","publish_status":"0","recid":"2000726","relation_version_is_last":true,"title":["The overlap of accessory virulence factors and multidrug resistance among clinical and surveillance Klebsiella pneumoniae isolates from a neonatal intensive care unit in Nepal: a single-centre experience in a resource-limited setting"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2024-04-16T01:08:35.458128+00:00"}