| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-13 |
| タイトル |
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|
タイトル |
Ionizing radiation induces mild and dose-independent damage to mitochondria in newt cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ionizing radiation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mitochondrial fission |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mitochondrial membrane potential |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mitochondrial superoxide |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Pleurodeles waltl |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hasan, Md Mahmudul
Kawabata, Tsuyoshi
Yan, Chen
Sekiya, Reiko
Goto, Shinji
Urata, Yoshishige
Li, Tao-Sheng
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| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
In addition to remarkable regenerative abilities, newts demonstrate a heightened tolerance to radiation compared to mammals. Mitochondria play profound role in cell survival when cells undergo environmental stresses. Thus, our study sought to elucidate the impact of ionizing radiation (IR) on the mitochondria of a newt model Pleurodeles waltl. Primary cells derived from limb tissue of P. waltl were exposed to 0, 5, 10, or 15 Gy X-ray and analyzed at 24h post-irradiation (PIR). Analysis using MitoTracker Red labeling revealed a maximal (p < 0.001) in mitochondrial fission in cells exposed to 5 Gy IR, while mitochondrial fission in cells exposed to 10 and 15 Gy IR was comparable (p < 0.01). Mitochondrial superoxide levels increased in a reverse dose-dependent manner; notably, cells treated with 5 Gy IR produced significantly (p < 0.05) higher mitochondrial superoxide. Mitochondrial membrane potential (ΔΨm) decreased significantly (p < 0.01) with similar extent across all IR-treated groups. Though ΔΨm declined, the ATP content was not changed due to IR. Result from the MTT assay indicated no impairment in mitochondrial activity. Cell counting data suggest negligible impact of IR on viability of cells; however, the phase contrast imaging revealed senescent like morphology of cells. Taken together, cells of P. waltl show mild changes in morphology and function of the mitochondria in response to IR, but seem highly tolerant. |
|
言語 |
en |
| 書誌情報 |
en : Experimental Cell Research
巻 448,
号 2,
p. art. no. 114575,
発行日 2025-04-29
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| 出版者 |
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|
出版者 |
Elsevier Inc. |
|
言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00144827 |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.yexcr.2025.114575 |
| 権利 |
|
|
権利情報 |
© 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Experimental Cell Research, 448(2), art. no. 114575; 2025 |
|
言語 |
en |
ECR448_114575.pdf (6.4 MB)
