| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-19 |
| タイトル |
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|
タイトル |
Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Chemically induced liver progenitor |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Transplantation |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Liver regeneration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Albumin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
TRAIL |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Fukumoto, Masayuki
Soyama, Akihiko
Miyamoto, Daisuke
Hara, Takanobu
Matsushima, Hajime
Imamura, Hajime
Yamashita, Mampei
Adachi, Tomohiko
Kanetaka, Kengo
Eguchi, Susumu
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We have explored chemically induced liver progenitors (CLiP) as a potential therapeutic agent for liver diseases because their proliferative capacity in vitro. Therefore, we hypothesized the potential treatment with CLiP transplantation (Tx) could be effective and be enhanced with liver regenerative stimulus for metabolic liver diseases. Methods: Male Sprague Dawley rats were used for mature hepatocytes (HEP) isolation to induce CLiP using 3 small molecules cocktail (ROCK-inhibitor, TGF beta-1 inhibitor, GSK3 inhibitor). Harvested rat CLiP (1 × 106) were transplanted to the posterior lobe of the liver (30 % of whole liver) of Nagase analbuminemic rats (NAR) through portal vein injection. One week later, portal venous branch to non-transplanted lobe was ligated to induce liver regeneration in CLiP bearing liver lobe (portal branch ligation: PBL). Only CLiP Tx or HEP Tx of same dose were used as control groups. Serial measurement of serum albumin level was measured by ELISA method. All NARs were subject to immunosuppression therapy using tacrolimus s.q. 3 days per week. Results: After HEP Tx especially with PBL, serum albumin level was significantly elevated. However, even with PBL, CLiP Tx group did not show significant increase in serum albumin. CLiP were surrounded by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) positive cells in portal veins suggesting that CLiP were exposed to the risk of innate immunity. Conclusion: Although CLiP respond to growth factors and proliferate better than HEP in vitro, their behavior in vivo in response to liver regenerative stimulus were not similar to in vitro probably because of less immaturity of receptors and/or high immunogenicity as compared to HEP. |
|
言語 |
en |
| 書誌情報 |
en : Regenerative Therapy
巻 30,
p. 1-8,
発行日 2025-05-09
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| 出版者 |
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出版者 |
Elsevier BV |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
23523204 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.reth.2025.04.015 |
| 権利 |
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|
権利情報 |
© 2025 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Regenerative Therapy, 30, pp.1-8; 2025 |
|
言語 |
en |
RT30_1.pdf (1.6 MB)
