| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-05-19 |
| タイトル |
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|
タイトル |
Antifibrotic effects of IL-12-treated natural killer cells on collagen type I regulation in lung fibroblasts |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Idiopathic pulmonary fibrosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Interleukin-12 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lung fibroblasts |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NK cells |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Okuno, Daisuke
Akiyama, Yoshiko
Sakamoto, Noriho
Tokito, Takatomo
Yura, Hirokazu
Kido, Takashi
Ishimoto, Hiroshi
Ishimatsu, Yuji
Takemoto, Shinnosuke
Takazono, Takahiro
Okamura, Haruki
Nishino, Tomoya
Tanaka, Yoshimasa
Mukae, Hiroshi
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| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Idiopathic pulmonary fibrosis is a chronic fibrotic lung disease with limited treatment options, making the development of new therapies crucial. We previously demonstrated that γδ T cells, a subset of immune effector cells, exhibit antifibrotic properties. We have also shown that natural killer (NK) cells, another class of immune effectors, can be efficiently expanded in culture using interleukin-2 (IL-2) and interleukin-18 (IL-18). This study examined the effects of interleukin-12 (IL-12)-stimulated NK cells, expanded with IL-2 and IL-18, on type I collagen and α-smooth muscle actin (αSMA) expression in pulmonary fibroblasts. IL-12-stimulated human NK cells exhibited reduced cytotoxicity toward pulmonary fibroblasts while retaining their proliferative capacity. Co-culture of IL-12-stimulated NK cells with fibroblasts significantly suppressed type I collagen and αSMA expression, even without direct cell contact, indicating the involvement of soluble factors. Supernatants from IL-12-stimulated NK cells partially inhibited the expression of these antifibrotic factors, suggesting a dual mechanism: direct cell-cell interaction and soluble factor secretion. Interferon-γ (IFN-γ) in the supernatant significantly increased, and neutralizing anti-IFN-γ monoclonal antibody partially reversed type I collagen and αSMA suppression. Similarly, IL-12-stimulated murine NK cells suppressed type I collagen in mouse pulmonary fibroblasts. These findings suggest that IL-12-stimulated NK cells inhibit the expression of fibrosis-associated molecules via contact-dependent and -independent mechanisms, supporting their potential for adoptive cell therapy in pulmonary fibrosis. |
|
言語 |
en |
| 書誌情報 |
en : Experimental Cell Research
巻 449,
号 1,
p. art. no. 114584,
発行日 2025-05-12
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| 出版者 |
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|
出版者 |
Elsevier Inc |
|
言語 |
en |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00144827 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.yexcr.2025.114584 |
| 権利 |
|
|
権利情報 |
© 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Experimental Cell Research, 449(1), art. no. 114584; 2025 |
|
言語 |
en |
ECR449_114584.pdf (2.2 MB)
