| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-06-03 |
| タイトル |
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タイトル |
Evaluating Signal Peptide Efficiency for Extracellular Protein Secretion for mRNA Vaccine Design |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
signal peptide |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
protein secretion |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
mRNA vaccine |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cystatin S |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
immunogenicity |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Sato, Shinya
Minagawa, Naoki
Hirata, Yuro
Sasaki, Masanori
Okamoto, Takumi
Kamiya, Mariko
Matsuhisa, Koji
Kawakami, Shigeru
Kaneko, Masayuki
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
mRNA vaccines have emerged as promising platforms for the prevention of infectious diseases and cancer treatment. The antigenic protein has a signal peptide added to the N-terminus for extracellular secretion. However, it remains unclear whether the optimization of signal peptides has been sufficiently compared and examined for antigen protein secretion and immunogenicity. This study investigated the effects of various signal peptides on the extracellular secretion of a model protein, NanoLuc luciferase (Nluc), in different cell lines. We compared the secretion efficiency of Nluc fused to artificial (#38 and #34) and natural signal peptides (cystatin S, lactotransferrin, and tissue plasminogen activator) in human embryonic kidney 293, C2C12, and HepG2 cells. Luciferase assays and Western blot analysis revealed that the cystatin S signal peptide consistently induced the highest secretion of Nluc among all cell types tested. Notably, the cystatin S signal peptide outperformed previously reported tissue plasminogen activator signal peptides in terms of secretion efficiency. Furthermore, we observed no correlation between Nluc secretion and mRNA expression levels for each signal peptide, suggesting that enhanced secretion was not attributable to increased transcription. Our findings highlight the potential of the cystatin S signal peptide in enhancing the extracellular secretion of antigenic proteins in mRNA vaccines by improving the efficiency of protein translation. |
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言語 |
en |
| 書誌情報 |
en : Biological and Pharmaceutical Bulletin
巻 48,
号 5,
p. 706-712,
発行日 2025-05-24
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| 出版者 |
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出版者 |
Pharmaceutical Society of Japan |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0918-6158 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1248/bpb.b25-00155 |
| 権利 |
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権利情報 |
© 2025 Author(s). This is an open access article distributed under the terms of Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/). Published by The Pharmaceutical Society of Japan. |
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言語 |
en |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
|
内容記述 |
Biological and Pharmaceutical Bulletin, 48(5), pp.706-712; 2025 |
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言語 |
en |
BPB48_706.pdf (888 KB)
