| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-07-08 |
| タイトル |
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タイトル |
Improved RSV Neutralization Assay Using Recombinant RSV Expressing Reporter Fluorescent Protein |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
RSV |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
|
主題 |
recombinant virus |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
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主題 |
fluorescent protein |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neutralization assay |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Yamagata, Yutaro
Toizumi, Michiko
Eleouet, Jean-Francois
Rameix-Welti, Marie-Anne
Takeda, Makoto
Yoshida, Lay-Myint
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Human respiratory syncytial virus (RSV) causes acute respiratory illness, attributing to deaths among young children and older adults worldwide. RSV neutralization assay is an important tool to measure RSV neutralization antibody that can prevent infection and severe complication of RSV. Conventional RSV neutralization assays have some limitations of speed and cost, especially for expensive kits, reagents or instruments required for detection. To solve this problem, this paper describes an improved simple and economical RSV neutralization assay protocol using recombinant RSV (rRSV) expressing reporter fluorescent protein to measure RSV growth as reporter activity with plate reader. The condition of 3 days culture demonstrated sufficient fluorescent activity even when small amounts of rRSV were used to inoculate Hep-2 cells. In addition, white 96-well cell culture plate showed better stable reporter activities than black plate. Furthermore, RSV neutralization assay protocol using rRSV-reporter fluorescent protein demonstrated similar signal detection capacity for RSV antibody titer detection compared to other protocols, such as rRSV-Luciferase and ELISA assay. The new RSV neutralization assay protocol can be applied to RSV antibody titration of numerous samples necessary for RSV surveillance or antiviral testing. |
|
言語 |
en |
| 書誌情報 |
en : Methods and Protocols
巻 8,
号 3,
p. art. no. 60,
発行日 2025-07-04
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| 出版者 |
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出版者 |
MDPI |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2409-9279 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.3390/mps8030060 |
| 権利 |
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権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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言語 |
en |
| 著者版フラグ |
|
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Methods and Protocols, 8(3), art. no. 60; 2025 |
|
言語 |
en |
MP8_60.pdf (1.1 MB)
