| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-07-08 |
| タイトル |
|
|
タイトル |
Healed Perforated Corneal Ulcers in Human |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
perforated corneal ulcer |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
healing |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
myofibroblasts |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
keratocytes |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
migration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
remodeling |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Mohamed, Yasser Helmy
Uematsu, Masafumi
Kusano, Mao
Suzuki, Keiji
Oishi, Akio
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
This study investigates the pathophysiological process of healed perforated corneal ulcers (HPCUs) in humans. All subjects underwent keratoplasty due to opacities or leakage from HPCUs. Half of each specimen was fixed with 4% glutaraldehyde for transmission electron microscope (TEM) examination. The other half was fixed in 10% formaldehyde for immunofluorescence (IF) examination. TEM identified layered structures with two cell types (polygonal and elongated) connected by gap or adherent junctions during early stage of healing. Both apoptotic and mitotic changes were found in both types of cells. There were no endothelial cells or Descemet’s membrane (DM) present in early stage of healing. During the intermediate stage, the healed area comprised three layers: epithelium, Bowman’s layer, and stroma, with an increase in stromal collagen. Later, adjacent endothelial cells crept in, forming DM and completing the cornea’s 5-layer structure. IF examinations revealed that vimentin+ and α-smooth muscle actin (αSMA)+ myofibroblasts gathered around the damaged site. Proliferating cell nuclear antigen+ cells, which indicated cell proliferation, were found in both cells. Anti-phospho-histone H2AX antibodies were found in some epithelial cells. CK14-positive cells were only found in superficial polygonal cells. Corneal wound healing is a complex process that includes apoptosis, cell migration, mitosis, differentiation, and extracellular matrix remodeling. |
|
言語 |
en |
| 書誌情報 |
en : Life
巻 15,
号 6,
p. art. no. 939,
発行日 2025-06-11
|
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2075-1729 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3390/life15060939 |
| 権利 |
|
|
権利情報 |
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
|
言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Life, 15(6), art. no. 939; 2025 |
|
言語 |
en |
L15_939.pdf (7.4 MB)
