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Characteristics of chemically induced liver progenitors derived from a pig model of metabolic dysfunction-associated steatotic liver disease
http://hdl.handle.net/10069/0002002616
http://hdl.handle.net/10069/00020026167aaaf9d5-6858-424e-8cc0-3a12313c71ac
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ISYK1710_Fukumoto.pdf (2.5 MB)
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| アイテムタイプ | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2025-07-11 | |||||||
| タイトル | ||||||||
| タイトル | Characteristics of chemically induced liver progenitors derived from a pig model of metabolic dysfunction-associated steatotic liver disease | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
福本, 将之
× 福本, 将之
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| 著者別名 | ||||||||
| 姓名 | Fukumoto, Masayuki | |||||||
| 言語 | en | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | We previously reported the efficacy of chemically induced liver progenitors (CLiP) as a source of cells for transplantation in patients with liver disease. This study aimed to characterize CLiP derived from steatotic livers using a pig model for future clinical applications. Livers were removed from miniature pigs with diet-induced steatosis and normal livers by laparoscopic hepatectomy. Mature hepatocytes (MH) isolated from the livers of each group were cultured in differentiation medium composed of Y-27632, A-83-01, and CHIR99021 (YAC medium). The characteristics of CLiP, including liver-specific function, proliferative capacity in vivo, and extracellular vesicles (EVs) production, were evaluated. Although CLiP in both groups expressed hepatic progenitor cell markers (Epithelial cell adhesion molecule and Trophoblast cell surface antigen 2), the proliferative potential was higher for the disease group than the healthy group. In contrast, markers of functional MH after re-differentiation were only detected in the healthy group. Both groups showed high cell viability and the ability to differentiate into albumin-positive cells in vivo. EVs counts were lower in disease-derived CLiP than in the normal group; however, there were no differences in microRNA expression within EVs. Using a pig model, CLiP was successfully produced from a liver that reproduced steatotic liver disease. Although there were slightly fewer EVs from CLiP in the disease group than in the normal liver group, the in vivo proliferative capacity of CLiP was high. Therefore, CLiP induced in the steatotic liver are a promising source for cell therapy in patients with liver disease. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1710号 学位授与年月日:令和7年6月4日 | |||||||
| 言語 | ja | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Author: Masayuki Fukumoto, Daisuke Miyamoto, Akihiko Soyama, Takanobu Hara, Yasuhiro Maruya, Peilin Li, Hajime Matsushima, Kazushige Migita, Takahiro Enjoji, Hanako Tetsuo, Takuro Fujita, Mampei Yamashita, Hajime Imamura, Tomohiko Adachi, Kengo Kanetaka, Takahiro Ochiya, Susumu Eguchi | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: PLOS ONE, 19(12), art. no. e0313312; 2024 | |||||||
| 言語 | en | |||||||
| 書誌情報 |
en : PLOS ONE 巻 19, 号 12, p. art. no. e0313312, 発行日 2025-06-04 |
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| ISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 1932-6203 | |||||||
| DOI | ||||||||
| 関連タイプ | isIdenticalTo | |||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1371/journal.pone.0313312 | |||||||
| 権利 | ||||||||
| 権利情報 | © 2024 Fukumoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||
| 言語 | en | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | 脂肪性肝疾患モデルブタ由来CLiP（化学的に誘導した肝前駆細胞）の特徴 | |||||||
| 言語 | ja | |||||||
| 出版者 | ||||||||
| 出版者 | Public Library of Science | |||||||
| 言語 | en | |||||||
| 関係URI | ||||||||
| 識別子タイプ | HDL | |||||||
| 関連識別子 | http://hdl.handle.net/10069/0002002530 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(医学) | |||||||
| 言語 | ja | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17301 | |||||||
| 学位授与機関名 | Nagasaki University (長崎大学) | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2025-06-04 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲医歯薬第1710号 | |||||||
| 学位の種類 | ||||||||
| 言語 | ja | |||||||
| 値 | 課程博士 | |||||||
| 引用 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Nagasaki University (長崎大学), 博士(医学) (2025-06-04) | |||||||
